摘　要：即时检验（POCT）是指在患者现场或附近进行的一类诊断测试（也称为床边测试），能够通过使用便携式诊断设备在短时间内获得准确的结果，避免将样本送往医学实验室。随着生物化学分析和微流体检测技术的发展，POCT已被广泛用于诊断和监测患者的疾病和健康状况。微流控纸基分析设备（µPADs）因其简单、用户友好、快速准确的结果读取和低成本而在POCT中广受欢迎。在过去的几十年里，一些µPADs已经成功商业化应用，显示出很好的发展前景。本文简要讨论了µPADs的主要类型、制备方法及其检测原理，并列举了几个具有代表性的例子，描绘了µPADs的未来发展前景。
关键词：即时检验；微流控纸基分析设备；传感器；个性化医疗；便携式诊断设备
中图分类号：TP212                         文献标志码：A              DOI：10.3969/j.issn.1007-7146.2025.01.001

Abstract: Point-of-care testing (POCT) refers to a category of diagnostic tests that are performed at or near to the site of the patients (also called bedside testing) and is capable of obtaining accurate results in a short time by using portable diagnostic devices, avoiding sending samples to the medical laboratories. It has been extensively explored for diagnosing and monitoring patients’ diseases and health conditions with the assistance of development in biochemistry and microfluidics. Microfluidic paper-based analytical devices (µPADs) have gained dramatic popularity in POCT because of their simplicity, user-friendly, fast and accurate result reading and low cost. Several µPADs have been successfully commercialized and received excellent feedback during the past several decades. This review briefly discusses the main types of µPADs, preparation methods and their detection principles, followed by a few representative examples. The future perspectives of the development in µPADs are also provided.
Key words: point-of-care testing; microfluidic paper-based analytical devices; sensor; personalized medical treatment; portable diagnostic equipment
CLC number: TP212                     Document code: A                      DOI: 10.3969/j.issn.1007-7146.2025.01.001
（Acta Laser Biology Sinica, 2025, 34(1): 001-011）