（徐州医科大学附属医院，徐州 221000）
摘　要：急性胰腺炎（AP）是需要紧急住院治疗的最常见的胃肠道疾病之一，其发病率呈上升趋势。本研究旨在评估恩格列净（EMPA）是否可通过抑制细胞凋亡来缓解大鼠AP。采用随机数表法将18只SPF级SD雄性大鼠分为对照组（CON组）、AP组及AP+EMPA组，每组6只。大鼠模型建立成功后采集血液及胰腺标本。检测的血清学指标有淀粉酶（AMY）、血糖（GLU）、肿瘤坏死因子-α（TNF-α）以及白介素-6（IL-6）水平。使用蛋白质印迹法检测大鼠胰腺组织中Caspase-3以及Bcl2的表达水平，使用免疫组化及免疫荧光检测大鼠胰腺组织中Caspase-3的表达水平。石蜡包埋胰腺组织进行苏木精-伊红（HE）染色以及病理评分，采用单因素方差分析对数据进行统计学处理。研究发现：AP组大鼠的血清AMY、GLU、炎症因子水平及病理评分较CON组升高；胰腺组织细胞凋亡相关分子Caspase-3较CON组升高，Bcl2的表达水平较CON组降低（P<0.05），而EMPA的治疗可以逆转上述现象。综上所述，EMPA可有效改善AP大鼠模型的胰腺组织病理变化，这种保护作用可能与抑制细胞凋亡有关。本文旨在探究EMPA在AP中的潜在治疗作用及其分子机制，为该疾病的有效防治提供新思路。
关键词：急性胰腺炎；细胞凋亡；Caspase-3；恩格列净；大鼠模型
中图分类号：R576                        文献标志码：ADOI：10.3969/j.issn.1007-7146.2024.06.009

(Affiliated Hospital of Xuzhou Medical University, Xuzhou 221000, China)
Abstract: Acute pancreatitis（AP） is one of the most common gastrointestinal diseases requiring urgent hospitalization, and its incidence is on the rise. This study aims to evaluate whether empagliflozin (EMPA) can alleviate AP in rats by inhibiting apoptosis. Eighteen SPF-grade SD male rats were randomly divided into three groups using a random number table method: control group (CON group), AP group, and AP+EMPA group, with six rats in each group. After successful establishment of the rat models, blood and pancreatic samples were collected. The serum markers tested included amylase (AMY), glucose (GLU), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) levels. Western blot analysis was used to detect the expression levels of Caspase-3 and Bcl2 in the pancreatic tissue, while immunohistochemistry and immunofluorescence were used to assess Caspase-3 expression in the pancreatic tissue. Pancreatic tissues were paraffin-embedded for hematoxylin-eosin (HE) staining and pathological scoring. One-way ANOVA was employed for statistical analysis of the data. The study found that the AP group had elevated serum AMY, GLU, inflammatory cytokine levels, and pathological scores compared to the CON group. In the pancreatic tissue, the apoptosis-related molecule Caspase-3 was elevated, while the expression level of Bcl2 was reduced compared to the CON group (P<0.05). However, EMPA treatment could reverse these changes. In conclusion, EMPA effectively ameliorates the pathological changes in the pancreatic tissue in a rat model of AP, and this protective effect may be related to the inhibition of apoptosis. This article aims to explore the potential therapeutic effects and molecular mechanisms of EMPA in AP, providing new insights for the effective prevention and treatment of this disease.
Key words: acute pancreatitis; apoptosis; Caspase-3; empagliflozin; rat model
（Acta Laser Biology Sinica, 2024, 33(6): 550-556）