（1. 南京工业大学柔性电子（未来技术）学院，南京 211816；2. 上海中航光电子有限公司，上海 200245）
摘　要：为了解决传统明场显微无法表征透明无染色大尺寸样品形貌的难题，本文提出了一种基于双透镜的暗场显微成像系统。系统主要包括激光器、空间光调制器（SLM）、双透镜、电荷耦合器件（CCD）。由SLM调制产生的环形涡旋光作为照明光，被前透镜聚焦到样品表面，样品被照明后，携带有样品高频信息的散射光被后透镜接收用于物体的暗场成像。试验中先后用木本双子叶植物椴树茎切片和南瓜茎切片作为试验样本，分别对比了两种切片的明暗场成像，结果表明，一种基于双透镜的自建暗场显微成像系统可以提高成像的分辨率和对比度，供透明无染色大尺寸样品形貌表征借鉴并推广使用。
关键词：双透镜；涡旋光；暗场成像；生物样品；形貌表征
中图分类号：O439                              文献标志码：ADOI：10.3969/j.issn.1007-7146.2024.06.006

(1. School of Flexible Electronics (Future Technologies), Nanjing Tech University, Nanjing 211816, China; 2. Shanghai AVIC Optoelectronics Co., Ltd, Shanghai 200245, China)
Abstract: To address the challenge of characterizing the morphology of large transparent and unstained samples that traditional bright-field microscopy cannot solve, a dark-field imaging system based on two-lens is proposed. The system primarily consists of a laser, a spatial light modulator, two lenses, and a charge-coupled device (CCD) camera. Ring-shaped vortex light modulated by the spatial light modulator serves as the illumination, focused onto the sample surface by the front lens. After illumination, the scattered light carrying high-frequency sample information was collected by the rear lens for dark-field imaging of the object. Experimental comparisons were conducted using sections of dicotyledonous wood and pumpkin stems as samples. The results indicated that a self-built dark-field microscopy system based on two-lens can enhance imaging resolution and contrast, offering a valuable approach for morphology characterization of large transparent and unstained samples.
Key words: two lens; vortex beam; dark field imaging; biological samples; morphological characterization
（Acta Laser Biology Sinica, 2024, 33(6): 523-529）