（1. Carbon Negative Synthetic Biology for Biomaterial Production from CO2，Campus for Research Excellence and Technological Enterprise，SJTU Asia-Pacific Graduate Institute，新加坡 138602；2. 上海交通大学生命科学技术学院，代谢与发育科学国际联合合作实验室，微生物代谢国家重点实验室，上海 200240）
摘　要：负碳合成生物学技术旨在直接捕获二氧化碳，实现生物制造，不仅可以解决传统生物制造中的非粮原料替代问题，还可助力“碳中和”和“碳达峰”，具备经济、社会、环境等多重效益。蓝藻能利用光能将二氧化碳转化为生物质，是负碳合成生物学技术中理想的底盘细胞。2019年报道的聚球藻（Synechococcus sp.）PCC 11901能够耐受高盐和高光胁迫，生长快且生物量大，有多套遗传操作系统可用，因此成为了负碳合成生物学底盘细胞中的一颗新星。本文综述了PCC 11901的研究进展，包括形态学、生理生化特性、基因组特征、开发的遗传操作工具以及改造PCC 11901后合成的化合物，旨在为未来PCC 11901的相关研究提供理论参考。
关键词：蓝藻；PCC 11901；生长与生物量；遗传操作；负碳合成生物学
中图分类号：Q81                                  文献标志码：ADOI：10.3969/j.issn.1007-7146.2024.06.002

(1. Carbon Negative Synthetic Biology for Biomaterial Production from CO2, Campus for Research Excellence and Technological Enterprise, SJTU Asia-Pacific Graduate Institute, Singapore 138602, Singapore; 2. State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic and Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China)
Abstract: Carbon-negative synthetic biology aims to directly capture CO2 to produce bio-products. This technique not only solves the “food vs. fuel” dilemma in traditional biological production, but also achieves CO2 peaking and carbon neutrality, therefore, it has obvious economic, ecological, and social benefits. Cyanobacteria, which use light energy to convert CO2 into biomass, are ideal chassis cells in CNSB. The newly discovered Synechococcus sp. PCC 11901 is a robust cyanobacterial strain with high resistance to salt and light, fast growth and big biomass, and multiple genetic toolbox available, representing a rising star among the cyanobacterial chassis cells. This review summarized the research progress in PCC 11901, including its morphology, biochemical and physiological features, genomic characteristics, available genetic toolboxes, and bio-products derived from engineered PCC 11901, with the aim of providing theoretical references for future research related to PCC 11901.
Key words: cyanobacterium; Synechococcus sp. PCC 11901; growth and biomass; genetic manipulation; carbon-negative synthetic biology
（Acta Laser Biology Sinica, 2024, 33(6): 489-495）