摘　要：多杀菌素是由刺糖多孢菌产生的次级代谢产物，具有绿色、高效、杀虫谱广等特点，是当前国际上生物杀虫农药的重点研究对象。多杀菌素及其类似物丁烯基多杀菌素的特殊结构决定了其杀虫独特的作用机理。当前，为满足大规模工业化生产的需求，研究人员致力于提高多杀菌素及丁烯基多杀菌素的产量，其中应用合成生物学技术在提高产量上取得了突出成就。本文综述了国内外通过合成生物学相关技术调控代谢途径以提高多杀菌素和丁烯基多杀菌素产量的研究，重点从底盘细胞改造与优化、合成生物系统构建、基因路线改造、代谢网络调控等方面进行了阐述，并提出和探讨了利用合成生物学技术进一步提高多杀菌素产量的技术策略，同时对未来的研究方向进行了展望，为利用合成生物学技术促进多杀菌素的生物合成提供了新的研究策略。
关键词：多杀菌素；刺糖多孢菌；合成生物学；基因编辑；代谢途径调控
中图分类号：Q81                                 文献标志码：ADOI：10.3969/j.issn.1007-7146.2024.05.002

Abstract: Spinosad, a secondary metabolite produced by Saccharopolyspora spinosa, has garnered significant attention globally due to its environmentally friendly and broad-spectrum insecticidal properties. The unique structure of spinosad and its analog, butenyl-spinosyn, underlies their distinctive mode of action. To meet the demands of large-scale industrial production, research teams worldwide have been striving to enhance the yields of spinosad and butenyl-spinosyn, with remarkable achievements accomplished through the application of synthetic biology techniques. This review summarizes the research conducted domestically and internationally on the regulation of metabolic pathways at various levels using synthetic biology techniques to improve the production of spinosad and butenyl-spinosyn. It focuses on four key aspects: chassis cell modification and optimization, multi-omics analysis, genetic circuit modification, and metabolic network regulation. Furthermore, it provides an outlook on future research directions in this field, and provides a new research strategy to promote the biosynthesis of spinosad by synthetic biology technology.
Key words: spinosad; Saccharopolyspora spinosa; synthetic biology; gene editing; metabolic pathway regulation
（Acta Laser Biology Sinica, 2024, 33(5): 400-407）