摘　要：探究安罗替尼干预对人肺鳞癌NCI-H226细胞糖酵解、增殖、克隆形成、迁移的影响及作用机制，为肺癌的体外试验提供参考依据。将人肺鳞癌NCI-H226细胞分为对照组（不做干预）、10、20、40 μmol/L安罗替尼组和阳性药物组（20 μg/mL顺铂）。用活细胞计数（CCK-8）、5-乙炔基-2′脱氧尿嘧啶核苷（EdU）、平板克隆形成、乳酸检测试剂盒、葡萄糖检测试剂盒、Transwell小室、实时荧光定量PCR（RT-qPCR）及蛋白免疫印迹（Western blotting）法对增殖活性、增殖率、克隆形成率、乳酸含量、葡萄糖消耗水平、细胞迁移数及上皮细胞间质化（EMT）相关因子表达水平进行测定。对照组、10、20、40 μmol/L安罗替尼组、阳性药物组NCI-H226细胞增殖活性、增殖率、克隆形成率、乳酸含量、葡萄糖消耗水平及N-钙黏蛋白（N-cadherin）、波形蛋白（vimentin）和纤维黏连蛋白（FN）mRNA和蛋白表达水平组间比较差异具有统计学意义（P<0.05）。20、40 μmol/L安罗替尼和阳性药物组细胞增殖活性高于对照组（P<0.05）。10、20、40 μmol/L安罗替尼组和阳性药物组细胞增殖率、克隆形成率、乳酸含量、葡萄糖消耗水平及N-cadherin、vimentin和FN mRNA和蛋白表达水平高于对照组（P<0.05）。安罗替尼可显著抑制人肺鳞癌NCI-H226细胞的糖酵解、增殖、克隆形成及迁移。

关键词：安罗替尼；肺癌；糖酵解；癌细胞增殖；克隆形成

中图分类号：R734.2                           文献标志码：ADOI：10.3969/j.issn.1007-7146.2024.01.010

Abstract: To investigate the effects and mechanism of anlotinib intervention on glycolysis, proliferation, colony formation and migration of human lung cancer NCI-H226 cells, to provide reference for in vitro test of lung cancer. Human lung cancer NCI-H226 cells were divided into control group which is without intervention, experimental group involving 10, 20, 40 μmol/L anlotinib, and positive control group involving 20 μg/mL cisplatin. The cell counting kit-8 (CCK-8), 5-acetylene-2′ deoxyuracil riboside (EdU) staining, plate clone formation, lactic acid detection kit, glucose detection kit, Transwell assay, RT-qPCR and Western blotting (WB) assays were used to detect proliferation viability, proliferation, colony-formation ability, lactic acid content, glucose consumption level, cell migration number and expression level of related factor levels. The proliferation activity, proliferation rate, clone formation rate, lactate content, glucose consumption level, N-cadherin, vimentin and fibronectin (FN) of NCI-H226 cells in control group, experimental group and positive drug group. There were significant differences in mRNA and protein expression levels between groups (P<0.05). The cell proliferation activity of 20 and 40 μmol/L anlotinib and positive drug groups was higher than that of control group (P<0.05). The cell proliferation rate, clone formation rate, lactate content, glucose consumption level and mRNA and protein expression levels of N-cadherin, vimentin and FN in the experimental group and the positive drug group were higher than those in the control group (P<0.05). Anlotinib can significantly inhibit glycolysis, proliferation, colony formation and migration of human lung cancer NCI-H226 cells.

Key words: anlotinib; lung cancer; glycolysis; proliferation of cancer cells; colony formation

（Acta Laser Biology Sinica, 2024, 33(1): 080-089）