摘　要：含PX结构域的丝氨酸/苏氨酸激酶基因（PXK）是一种多组织广谱表达的基因，在免疫相关疾病中发挥了重要作用。本研究的目的是通过多个数据库中的多个生物信息学方法分析PXK与急性髓细胞样白血病（AML）发生发展的相关性。利用UALCAN 数据库在线分析 PXK 在 AML 不同亚型中的表达情况；利用LinkedOmics网站分析PXK表达水平与AML临床病理特征的相关性；利用Kaplan-Meier Plotter数据库分析PXK在AML中mRNA的表达情况，并评估PXK在AML中对生存率的影响；利用String和GEPIA数据库分析PXK互作蛋白网络及表达相关性；利用quanTIseq算法分析PXK基因与肿瘤浸润免疫细胞相关性；利用“xCell”R包分析PXK基因表达水平与多个免疫检查点表达相关性；利用Metascape数据库对PXK及其共表达基因进行功能富集分析，并预测其生物学功能。结果显示，PXK在多种癌症肿瘤组织中较正常组织高表达，在AML中的表达水平显著上调，并对AML患者的各临床病理特征、生存率及预后产生影响，但高表达PXK的AML患者预后较好。PXK在AML组织中高表达，并与AML的发生发展密切相关，可能是AML诊断、治疗效果及预后评估的重要指标之一。
关键词：急性髓细胞样白血病；PXK；表达；预后
中图分类号：Q811.4                             文献标志码：A            DOI：10.3969/j.issn.1007-7146.2023.05.008

Abstract: PX domain containing serine/threonine kinase (PXK) is a multitissue, broad-spectrum expressed gene that plays an important role in immune-related diseases. The objective of this study was to analyze the relationship between PXK and the development of acute myelocytic leukemia (AML) by using bioinformatics methods in multiple databases. The UALCAN database was utilized to conduct an online analysis of PXK expression in various subtypes of AML. LinkedOmics was utilized to analyze the correlation between the expression level of PXK and the clinicopathologic features of AML. The Kaplan-Meier Plotter database was used to analyze the mRNA expression of PXK in AML and to evaluate the effect of PXK on survival in AML. PXK interacting protein network and expression correlation were analyzed by String and GEPIA databases. The correlation between PXK gene and tumor infiltrating immune cells was analyzed by quanTIseq algorithm, and “xCell” R package was used to analyze the correlation between PXK gene expression and multiple immune checkpoints. PXK and its co-expressed genes were functionally enriched in the Metascape database to anticipate their biological roles. The findings revealed that PXK was highly expressed in various cancer tumor tissues when compared to normal tissues, and the expression level of PXK in AML was significantly up-regulated, which had an effect on the clinicopathological features, survival rate, and prognosis of AML patients, whereas the prognosis of AML patients with high PXK expression was better. PXK is highly expressed in AML tissues and is closely related to the initiation and progression of AML, which may be one of the important indicators for the diagnosis, treatment and prognosis evaluation of AML.
Key words: acute myeloid leukemia; PXK; expression; prognosis
（Acta Laser Biology Sinica, 2023, 32(5): 450-459）