摘　要：为探究槲皮素对人软骨肉瘤细胞迁移、侵袭和上皮间质转化（EMT）的影响及对Janus激酶2/信号转导和转录启动因子3（JAK2/STAT3）信号通路的调控作用，体外培养人软骨肉瘤SW-1353细胞，用不同浓度槲皮素干预SW-1353细胞24 h，细胞计数试剂盒（CCK-8）法筛选出最适浓度（50.0 μmol/L）槲皮素进行后续试验。将细胞分为对照组（不做干预）、槲皮素组（50.0 μmol/L槲皮素）、阳性药物组（6.0 μmol/L阿霉素）、抑制剂组（50.0 μmol/L槲皮素＋50.0 μmol/L JAK2/STAT3通路抑制剂AG490）和激活剂组（50.0 μmol/L槲皮素＋0.5 μmol/L JAK2/STAT3通路激活剂colivelin），干预24 h后，用倒置显微镜、Transwell小室及Western blot法对细胞形态、迁移、侵袭及JAK2/STAT3通路和EMT相关蛋白的表达水平进行分析。试验结果显示：CCK-8法确定槲皮素最适作用浓度为50.0 μmol/L；与对照组比较，槲皮素组和阳性药物组细胞生长受到抑制，细胞迁移数、侵袭数、N-钙黏蛋白（N-cadherin）、波形蛋白（Vimentin）、纤连蛋白（FN）、p-JAK2和p-STAT3表达水平降低（P<0.05），E-钙黏蛋白（E-cadherin）表达水平升高（P<0.05）；与槲皮素组相比，抑制剂组上述指标变化趋势更为显著（P<0.05），激活剂组则扭转了这些指标的变化（P<0.05）。槲皮素可抑制人软骨肉瘤SW-1353细胞的生长、迁移、侵袭和EMT进程，其作用机制可能与阻滞JAK2/STAT3信号通路相关。
关键词：软骨肉瘤；槲皮素；JAK2/STAT3信号通路；迁移；侵袭
中图分类号：Q811.4                           文献标志码：A                       DOI：10.3969/j.issn.1007-7146.2023.03.007

Abstract: To investigate the effects of quercetin on human chondrosarcoma cell migration, invasion and epithelial interstitial transformation (EMT) and the regulatory role of Janus kinase 2/signal transduction and transcription promoter 3 (JAK2/STAT3) signaling pathway, human chondrosarcoma SW-1353 cells were cultured in vitro and treated with quercetin of different concentrations for 24 h. The optimal concentration of quercetin (50.0 μmol/L) was selected by cell counting kit (CCK-8) for subsequent experiments. The cells were divided into control group with no intervention, quercetin group with 50.0 μmol/L quercetin, positive control group with 6.0 μmol/L adriamycin, inhibitor group involving 50.0 μmol/L quercetin +50.0 μmol/L JAK2/STAT3 pathway inhibitor AG490, and activator group involving 50.0 μmol/L quercetin +0.5 μmol/L JAK2/STAT3 pathway activator colivelin. After 24 h of intervention, cell morphology, migration, invasion, and expression levels of JAK2/STAT3 pathway and EMT-related proteins were analyzed by inverted microscope, Transwell chamber and Western blot. The experimental results showed that the optimum concentration of quercetin was determined by CCK-8 method to be 50.0 μmol/L. Compared with the control group, the growth of quercetin group were positive control group were inhibited, the cell migration number, invasion number, expression levels of N-cadherin, Vimentin, fibronectin (FN), p-JAK2 and p-STAT3 in quercetin group and positive control group decreased (P<0.05), E-cadherin expression level increased (P<0.05); compared with quercetin group, the change trend of the above indexes in inhibitor group was more significant (P<0.05), while the change of these indexes in activator group was reversed (P<0.05). Quercetin can inhibit the growth, migration, invasion and EMT progression of human chondrosarcoma SW-1353 cells, possibly by blocking the JAK2/STAT3 signaling pathway.
Key words: chondrosarcoma; quercetin; JAK2/STAT3 signaling pathway; migration; invasion
（Acta Laser Biology Sinica, 2023, 32(3): 240-246）