摘　要：本文以具有亲疏水特点的磷脂分子为基质材料，采用薄膜水化法制备了一种同时包载脂溶性白藜芦醇（Res）和水溶性维生素C（VC）的纳米复合脂质体（Lip@Res/VC），并将其用于细胞抗氧化研究。在Lip@Res/VC的制备过程中，以Res和VC的包封率以及脂质体的粒径、多分散指数和电位为指标，考察了Res和VC的最佳投入量。研究结果表明，分别选择Res为4.0 mg和VC为3.5 mg的投入量，制备的Lip@Res/VC的水合粒径为（92.29±5.41）nm，Res和VC的包封率分别为（85.13±5.37）%和（64.73±5.51）%。体外1，1-二苯基-2-三硝基苯肼（DPPH）和2，2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸二胺盐（ABTS）自由基清除试验结果显示，Lip@Res/VC的抗氧化活性较只包载了Res的单一脂质体Lip@Res或只包载了VC 的单一脂质体Lip@VC均显著提高。通过选择正常肝细胞（L02细胞）为模式细胞，研究了Lip@Res/VC的细胞内抗氧化效果，结果显示，Lip@Res/VC具有清除细胞内活性氧（ROS）的能力，对过氧化氢诱导的L02细胞氧化损伤具有保护作用。
关键词：纳米复合脂质体；白藜芦醇；维生素C；活性氧；抗氧化
中图分类号：Q819　　　　　　文献标志码：A　　　　        DOI：10.3969/j.issn.1007-7146.2023.03.005

Abstract:  By using phospholipid molecules with hydrophilic and hydrophobic properties as matrix materials, the nanocomposite liposomes (Lip@Res/VC) containing both fat-soluble resveratrol (Res) and water-soluble vitamin C (VC) have been prepared through thin film hydration method and have been further used for cellular antioxidant studies. In the preparation of Lip@Res/VC, the optimal added amount of Res and VC were determined by evaluating the encapsulation rate of Res and VC, hydration particle size, polydispersal index and zeta potential of nanocomposite liposomes. The results showed that the hydration particle size of Lip@Res/VC was (92.29±5.41) nm and the encapsulation efficiency of Res and VC were (85.13±5.37)% and (64.73±5.51)%, when the added amount of Res was 4.0 mg and VC was 3.5 mg, respectively. The DPPH (2, 2-diphenyl-1-picrylhydrazyl) and ABTS ［2, 2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt］ free radical scavenging assay in vitro showed that the antioxidant activity of Lip@Res/VC was significantly higher than that of Lip@Res or Lip@VC. Furthermore, L02 cells were selected as model cells to study the intracellular antioxidant effect of Lip@Res/VC. The results showed that Lip@Res/VC is capable of removing intracellular reactive oxygen species (ROS) and protecting L02 cells from oxidative damage induced by hydrogen peroxide.
Key words: nanocomposite liposomes; resveratrol; vitamin C; reactive oxygen species; antioxidant
（Acta Laser Biology Sinica, 2023, 32(3): 226-233）