摘　要：随着生物工程技术的快速发展，细胞工程愈来愈受到重视。昆虫细胞培养技术作为生物工程的新领域，在现代生物学上具有重要的价值，其中以昆虫杆状病毒为载体，以昆虫细胞为受体的基因工程研究也取得了突破性的进展。体外培养的昆虫细胞可以高效地表达外源基因，且昆虫杆状病毒具有对人畜无害等特点，使得昆虫细胞培养技术广泛地应用于生物学、医药学和农业等各个领域。本文综述了昆虫细胞培养的研究进展，其中包括了体外培养昆虫细胞培养基的基础成分及无血清培养基的添加物、生物反应器大规模培养昆虫细胞和昆虫杆状病毒表达载体系统及其应用，为昆虫细胞无血清培养基的研发提供了理论依据。
关键词：昆虫细胞；杆状病毒；无血清培养；大规模培养
中图分类号：Q813.1+1                              文献标志码：A                       DOI：10.3969/j.issn.1007-7146.2023.03.002

Abstract: With the rapid development of the field of bioengineering, cell engineering has been paid more and more attention. Insect cell culture technology, as a new field of bioengineering, has important value in modern biology, among which the genetic engineering research using insect baculovirus as the carrier and insect cells as the receptor has also made breakthrough progress. Insect cells cultured in vitro can express foreign genes efficiently, and insect baculoviruses are harmless to humans and animals, which makes insect cell culture technology being widely used in various fields such as biology, medicine and agriculture. In this paper, the research progress of insect cell culture is reviewed, including the basic components of insect cell culture medium in vitro, the addition of unclear medium, the bioreactor for the large-scale culture of insect cellss and the vector system of insect baculovirus expression and its application. It provides a theoretical basis for the development of serum-free culture medium for insect cells.
Key words: insect cells; baculovirus; serum-free culture; mass cell culture
（Acta Laser Biology Sinica, 2023, 32(3): 200-207）