摘　要：硫是植物生长发育的一种必需营养元素，主要以SO42-的形式被植物吸收。硫酸盐转运蛋白（SULTR）在植物吸收与转运硫的过程中发挥着重要的作用。本研究鉴定得到10个黄瓜SULTR基因家族成员（CsaSULTR1;1~CsaSULTR4;1）。系统发育分析结果表明，黄瓜和拟南芥的SULTR可分为GROUP 1～4四个类群。大多数CsaSULTR基因含9～12个内含子，且亲缘关系相近的成员之间基因结构也十分相似。启动子顺式元件分析预测表明，CsaSULTR基因家族成员均包含与激素应答和胁迫相关的作用元件，表明它们可能在生长发育过程中发挥了主要作用。转录组数据表达分析也表明，CsaSULTR基因在各种组织间都存在着比较突出的表达特异性，且部分基因的表达量在霜霉病胁迫下表现出显著的改变。这些结果为下一步研究黄瓜SULTR基因的功能奠定了重要的基础。
关键词：黄瓜；SULTR基因家族；进化分析；转录组数据；霜霉病
中图分类号：Q751                               文献标志码：A                    DOI：10.3969/j.issn.1007-7146.2022.04.009

Abstract: Sulfur is an essential macronutrient for plant growth and development, and sulfate transporter (SULTR) plays key roles in sulfate (SO42-) uptake and transport in higher plants. In this study, 10 SULTR genes (CsaSULTR1;1～CsaSULTR4;1) were identified in cucumber. Phylogenetic analysis showed that SULTRs from cucumber and Arabidopsis could be divided into four groups (GROUP 1～4). Most CsaSULTR genes contained 9～12 introns, and closely related members shared similar gene structures. Promoter analysis revealed that CsaSULTR genes may be involved in responses to hormones and environmental stress. Expression profiling analysis by RNA-seq indicated that CsaSULTR genes have distinct expression patterns in different tissues, and some of them had significantly changed expression in response to downy mildew infection. Our results provide a better understanding for further functional analysis of CsSULTR genes in cucumber.
Keywords: cucumber; SULTR gene family; phylogenetic analysis; transcriptome data; downy mildew
（Acta Laser Biology Sinica, 2022, 31(4): 353-360）