摘　要：为了研究藻酸盐裂解酶（AlgL）对高黏液型肺炎克雷伯杆菌（HvKP）生物被膜和细菌因子RpoS基因的影响，从临床送检的痰液样本中分离出30株肺炎克雷伯杆菌（KP）。采用全自动快速微生物质谱检测系统VITEK进行菌种鉴定，通过药敏试验进行耐药分析，拉丝试验阳性的菌株确定为HvKP；采用半定量结晶紫染色法进行细菌生物被膜半定量检测；用聚合酶链反应（PCR）检测RpoS基因的表达情况。试验发现，AlgL对HvKP生物被膜的形成有显著性抑制作用。本研究为AlgL在临床上用于治疗HvKP提供了研究基础。
关键词：藻酸盐裂解酶；高黏液性肺炎克雷伯菌；获得性感染；细菌生物被膜；RpoS基因        
中图分类号：Q93                                   文献标志码：A                  DOI：10.3969/j.issn.1007-7146.2022.04.007

Abstract: In order to study the effect of alginate lyase (AlgL) on biofilm and bacterial factors RpoS gene of hypervirulent Klebsilla pneumoniae (HvKP), 30 strains of Klebsiella pneumoniae (KP) were isolated from clinical samples. They were identified by automatic rapid microbial mass spectrometry detection system VITEK, were done susceptibility tests for drug resistance analysis, the strains with positive wire drawing test were identified as HvKP. The bacterial biofilm was detected by semi quantitative crystal violet staining. The expression of RpoS gene was detected by polymerase chain reaction (PCR). AlgL can significantly inhibit HvKP biofilm, and the thickness of biofilm is positively correlated with the expression of RpoS gene, indicating that alginate lyase can inhibit the formation of HvKP biofilm. It provides a research basis for AlgL in the clinical treatment of HvKP.
Key words: alginate lyase; hypervirulent Klebsilla pneumoniae; acquired infection; bacterial biofilm; RpoS gene
（Acta Laser Biology Sinica, 2022, 31(4): 337-343）