摘　要：通过多种肿瘤数据库和生物信息学方法分析磷脂酶A2-Ⅶ（PLA2G7）的基因在宫颈癌中的表达与预后及免疫浸润的关系。利用cBioPortal、GEO、GEPIA2、TCGA数据库分析PLA2G7基因的遗传变异、表达情况及其与患者预后的关系。利用基因集富集分析（GSEA）和基因集变异分析（GSVA）方法研究TCGA中宫颈癌患者PLA2G7表达的生物学功能及参与的信号通路。通过TIMER数据库分析PLA2G7表达与免疫浸润细胞及其标记基因之间的相关性。结果显示：与正常宫颈组织相比，PLA2G7在宫颈肿瘤组织中高表达；PLA2G7的高表达显著富集在趋化因子信号通路、自然杀伤细胞介导的细胞毒性、T细胞受体信号通路等免疫相关通路上；PLA2G7高表达与总生存期（OS）的延长相关（log-rank P=0.030）；亚组分析表明，PLA2G7高表达在年老组、stageⅠ&stageⅡ和G3&G4中与较长的OS相关；PLA2G7表达与宫颈肿瘤组织中CD4+ T细胞、CD8+ T细胞、巨噬细胞、中性粒细胞和树突状细胞的浸润水平呈正相关，并与免疫细胞基因标记物CD86、CD115（CSF1R）、CD163、MS4A4A、CD11c（ITGAX）、CTLA4和TIM-3（HAVCR2）的表达存在强相关性。本研究表明，在宫颈癌患者中PLA2G7高表达可能是通过增强免疫细胞的募集信号通路、调节免疫浸润细胞的富集，从而改善宫颈癌患者的生存状况。这为PLA2G7作为宫颈癌诊断治疗及其预后评估的生物标志物提供了理论基础。
关键词：PLA2G7；宫颈癌；免疫浸润；预后
中图分类号：R737.33                        文献标志码：ADOI：10.3969/j.issn.1007-7146.2022.03.011

Abstract: To investigate the expression of phospholipase A2 groupⅦ (PLA2G7) gene in cervical cancer tissues by multiple tumor databases and bioinformatics methods, and to analyze the relationship between PLA2G7 and clinical prognosis and immune infiltration, we analyzed the genetic aberration， expression of PLA2G7 gene and its relationship with patient prognosis by using cBioPortal, GEO, GEPIA2, and TCGA databases. GSEA and GSVA were performed to evaluate the potential biological functions of PLA2G7. The correlations between PLA2G7 gene expression and immune infiltration and their marker genes were investigated via TIMER database. The results have indicated that compared with normal cervical tissues, PLA2G7 was higher in cervical cancer tissues, and high PLA2G7 expression were significantly enriched in immune-related pathways such as chemokine signaling pathway, natural killer cell-mediated cytotoxicity and T cell receptor signaling pathway. High expression of PLA2G7 was associated with prolonged OS (Log-rank P=0.030). Subgroup analysis revealed that high expression of PLA2G7 was related with better OS in older, stageⅠ&stageⅡ, and G3&G4 patients but not other subgroup patients. PLA2G7 expression was positively correlated with infiltrating levels of CD4+ T cells, CD8+ T cells, macrophages, neutrophils, and dendritic cells. And it was strongly correlated with immune cell markers CD86, CD115 (CSF1R), CD163, MS4A4A, CD11c (ITGAX), CTLA4 and TIM-3 (HAVCR2). The results of analysis showed that in cervical tumor tissues, the high expression of PLA2G7 may improve the survival of patients by enhancing the recruitment signaling pathway of immune cells and regulating the enrichment of immune infiltration cells, which provides a theoretical basis for the use of PLA2G7 as a biomarker for the diagnosis and treatment of cervical cancer and its prognostic assessment.
Key words: PLA2G7; cervical cancer; immune infiltration; prognosis
（Acta Laser Biology Sinica, 2022, 31(3): 268-277）