摘 　要：为了探讨核黄素对IL-17A-/-氟中毒小鼠肾脏纤维化的影响，选取6周龄野生型和IL-17A-/- C57BL/6J雄性小鼠各32只，分为对照组（0.9% NaCl）、氟化钠组（24 mg/kg NaF）、核黄素组（5 mg/kg 核黄素磷酸钠）、氟化钠+核黄素组（24 mg/kg NaF+5 mg/kg核黄素磷酸钠），每组8只。连续灌胃90 d后，对小鼠肾脏组织形态、纤维化程度、血清生化指标肌酐（Cr）、尿酸（UA）和尿素氮（BUN）进行检测。结果显示，核黄素能够减轻氟引起的小鼠肾脏组织形态学损伤和纤维化程度，不能改变氟导致的正常小鼠血清Cr、UA、BUN升高现象。这些结果表明，添加核黄素可以缓解IL-17A-/-小鼠氟中毒导致的肾脏纤维化，且核黄素在IL-17A-/-小鼠中的缓解效果要比正常小鼠更好。
关键词：氟中毒；核黄素；小鼠；肾脏纤维化；IL-17A
中图分类号：X503.22                          文献标志码：A                  DOI：10.3969/j.issn.1007-7146.2022.03.009

Abstract: To explore the effects of riboflavin on renal fibrosis in IL-17A-/- mice induced by fluoride, 32 wild-type and 32 IL-17A-/- 6-week-old C57BL/6J male mice were randomly divided into the control group (0.9% NaCl), sodium fluoride group (24 mg/kg NaF), riboflavin group (5 mg/kg riboflavin sodium phosphate), and sodium fluoride plus riboflavin group (24 mg/kg NaF + 5 mg/kg riboflavin sodium phosphate), 8 mice each group. Mice were treated by gavage for consecutive 90 days, then the renal morphological changes, fibrosis degree, and serum levels of Cr, UA, and BUN were detected. The results showed that riboflavin could reverse the degree of renal histological damage and fibrosis in mice caused by fluoride. Riboflavin did not alleviate the increase of serum Cr, UA and BUN levels induced by fluoride in wild type mice. The results suggested that riboflavin could alleviate the renal fibrosis degree induced by fluoride in wild type and IL-17A-/- mice, and IL-17A-/- mice presented better mitigation effect.
Key words: fluorosis; riboflavin; mouse; renal fibrosis; IL-17A
（Acta Laser Biology Sinica, 2022, 31(3): 256-260）