摘　要：基于生物信息学分析筛选出一段源于转录因子FOXM1的肿瘤抗原肽M1-10。通过淋巴细胞增殖、干扰素γ释放及细胞杀伤等试验，证实M1-10肽段可在小鼠体内诱导有效的免疫记忆，与HJURP、MELK、VEGFR1、VEGFR2来源的四种抗原肽联用可产生更好的效果。运用小鼠乳腺癌移植瘤模型和MMTV-PyMT原发乳腺癌模型开展M1-10单独或联合四种抗原肽的肿瘤预免疫试验，发现M1-10单独使用可发挥显著的抑瘤效果，联合四种抗原肽的抑瘤效果更强。总之，本研究开发了一种肿瘤抗原肽，该肽免疫原性强，单独或与其他肿瘤抗原肽联用时可实现有效的肿瘤免疫。该结论为FOXM1的抗肿瘤机制提供了进一步的见解。
关键词：肿瘤抗原肽M1-10；转录因子FOXM1；免疫记忆；肿瘤免疫；小鼠
中图分类号：Q730.3                            文献标志码：ADOI：10.3969/j.issn.1007-7146.2022.02.004

Abstract: Based on bioinformatics analysis, a transcription factor FOXM1-derived tumor antigen peptide M1-10 was screened out. Through the experiments of lymphocyte proliferation, interferon gamma release, and cell killing, we found that M1-10 peptide could stimulate the immune memory in vivo and produce better effects when combined with HJURP, MELK, VEGFR1, and VEGFR2-derived four antigens. Tumor pre-immunization experiments were carried out with the mouse models of grafted breast cancer and MMTV-PyMT primary breast cancer. Compared with the control group, M1-10 alone produced significant anti-tumor effects, and M1-10 plus four antigen generated stronger anti-tumor effects. In conclusion, the study developed the tumor antigen peptide M1-10 with a strong immunogenicity, which could result in effective tumor immunity when used alone or combined with other tumor antigen peptides. This conclusion provides further insights into the antitumor mechanism of FOXM1.
Key words: tumor antigen peptide M1-10; transcription factor FOXM1; immune memory; tumor immunity; mouse
（Acta Laser Biology Sinica, 2022, 31(2): 121-128）