摘　要：通过分析红光/近红外LED光照射治疗（LED光疗）对外周血单核细胞中疼痛相关炎症因子及趋化因子的表达影响，研究红光/近红外光生物治疗对疼痛的抑制作用。在用人单核细胞THP-1评价红光/红外光的安全剂量的基础上制备健康成年人外周血单核细胞（PBMC），进行红光/红外光照射处理后，用荧光定量PCR法检测疼痛相关炎症因子及趋化因子的表达变化。结果显示，经过红光/近红外LED光疗后，与对照组相比，PBMC的CXCL12、CXCL13、CX3CL1、CCL4、CCR2、IL-4、IL-6和MMP2基因表达水平明显下调，表明红光/近红外LED光疗可通过抑制PBMC中疼痛相关趋化因子及炎症因子表达发挥抗疼痛作用。此研究可为疼痛辅助治疗提供新的思路。
关键词：红光/近红外LED光照射治疗；外周血单核细胞；趋化因子；炎症因子；疼痛
中图分类号：R318.51                          文献标志码：A DOI：10.3969/j.issn.1007-7146.2022.02.003

Abstract: To study the inhibitory effect of red/near-infrared light irradiation therapy (LED phototherapy) on pain, the expression of pain related inflammatory factors and chemokines were analyzed in peripheral blood monocytes. Base on evaluating the safe dose of red light/infrared light with human monocyte THP-1, PBMC from peripheral blood monocytes of healthy adults were prepared. After red light/infrared light irradiation, the expression of pain related inflammatory factors and chemokines were detected by fluorescence quantitative PCR. After treatment with red/infrared light, the gene expression levels of CXCL12, CXCL13, CX3Cl1, CCl4, CCR2, IL-4, IL-6 and MMP2 in PBMC in the LED group were significantly down regulated compared with the control group. Red/near infrared light phototherapy can play an antipain role by inhibiting the expression of pain related chemokines and inflammatory factors in PBMC. This study may provide new ideas for pain adjuvant treatment.
Key words: red/near infrared light irradiation therapy; peripheral blood monocytes; chemokines; inflammatory factors; pain
（Acta Laser Biology Sinica, 2022, 31(2): 114-120）