摘　要：为明确大气CO2浓度升高对大豆糖代谢和脂肪代谢的影响，本研究以高油大豆品种“晋大70”为材料，利用控制气室设置CK（CO2浓度为400 μmol/mol）和EC（CO2浓度为600 μmol/mol）两个处理，大豆整个生育期均在控制气室内，在大豆鼓粒期利用便携式气体交换系统LI-6400测定光合参数，测定叶绿素含量、糖代谢和脂肪代谢相关指标，并在收获期测定产量指标。结果表明：CO2浓度升高显著提高叶绿素a、叶绿素b和总叶绿素含量，使大豆净光合速率和水分利用效率显著提高59.7%和63.2%；叶片中的蔗糖磷酸合成酶和异柠檬酸裂解酶活性在高CO2浓度下显著升高28.8%和215.0%，琥珀酸脱氢酶活性、乙酰辅酶A羧化酶和二酰基甘油酰基转移酶活性显著降低；CO2浓度升高使籽粒中可溶性糖含量、蔗糖含量、蔗糖合成酶和异柠檬酸裂解酶活性显著下降，但显著增加异柠檬酸脱氢酶（248.2%）、琥珀酸脱氢酶（75.0%）、乙酰辅酶A羧化酶活性（15.8%）；大豆的单株粒重、单株荚数和百粒重在CO2浓度升高条件下显著升高25.6%、23.4%和21.1%。研究表明，在高CO2浓度下，大豆鼓粒期叶片蔗糖代谢和磷酸戊糖途径代谢加强，三羧酸循环和脂肪代谢减弱，籽粒中蔗糖代谢减弱，磷酸戊糖途径、三羧酸循环和脂肪代谢加强。本研究结果将有助于了解CO2浓度升高条件下对大豆糖代谢和脂代谢的影响，为未来气候变化背景下大豆生产与管理提供理论依据。
关键词：CO2浓度；大豆；蔗糖代谢；三羧酸循环；脂肪代谢
中图分类号：S529                              文献标志码：ADOI：10.3969/j.issn.1007-7146.2022.01.010

Abstract: To investigate the effects of elevated CO2 concentration on sugar metabolism and fat metabolism of soybean. The high-oil soybean variety“Jinda-70”was used as material, and two treatments were set up in a controlled chamber: CK (CO2 concentration 400 μmol/mol) and EC (CO2 concentration 600 μmol/mol). The whole growth period of soybean was in the controlled gas chamber. The portable gas exchange system Li-6400 was used to measure the indexes of the photosynthetic parameters, chlorophyll content, glucose metabolism and fat metabolism at the bulking stage, and the yield indexes at the harvest stage were also measured. The results showed that the contents of chlorophyll a, chlorophyll b and total chlorophyll were significantly increased by increasing CO2 concentration, and the net photosynthetic rate and water usage efficiency of soybean also significantly increased by 59.7% and 63.2%. The activities of sucrose phosphate synthase and isocitrate lyase in leaves significantly increased by 28.8% and 215.0% under high CO2 concentration, while the activities of succinate dehydrogenase, acetyl-CoA carboxylase, and diacylglycerol acyltransferase significantly decreased. With the increase of CO2 concentration, soluble sugar content, sucrose content, sucrose synthase and isocitrate lyase activities were significantly decreased, while the activities of isocitrate dehydrogenase, succinic dehydrogenase and acetyl-CoA carboxylase were significantly increased by 248.2%, 75.0% and 15.8%. In addition, the seed weight per plant, pod number per plant, and 100-seed weight of soybean significantly increased by 25.6%、23.4% and 21.1% under increased CO2 concentration. In soybean leaf, sucrose metabolism and pentose phosphate pathway were strengthened, whereas tricarboxylic acid cycle and fat metabolism were weakened at seed filling stage in soybean. In soybean seed, pentose phosphate pathway, tricarboxylic acid cycle and fat metabolism were strengthened along with weakened sucrose metabolism. The results will help to understand the effects of elevated CO2 concentration on sugar metabolism and lipid metabolism in soybean, and provide theoretical basis for the production and management in soybean under climate change scenarios in the future.
Key words: CO2 concentration; soybean; sucrose metabolism; tricarboxylic acid cycle; fat metabolism
（Acta Laser Biology Sinica, 2022, 31(1): 070-078）