摘　要：本文将法夫酵母虾青素、β-胡萝卜素、海胆酮和三者的组合物作用于长波长紫外线（UVA）氧化损伤的人皮肤成纤维（HSF）细胞，以乳酸脱氢酶（LDH）活性和丙二醛（MDA）含量为指标评价类胡萝卜素对HSF细胞因紫外辐射而氧化损伤的保护作用。结果表明：单一类胡萝卜素及它们的组合均能显著降低LDH活性和MDA含量（P<0.05），对UVA导致的HSF细胞的氧化损伤具有保护作用；相同质量浓度下，虾青素降低LDH活性的能力优于β-胡萝卜素，对HSF细胞氧化损伤的保护效果较好。本研究为利用类胡萝卜素开发对UVA辐射氧化损伤具有保护作用的化妆品和保健品提供了理论依据。
关键词：虾青素；β-胡萝卜素；海胆酮；紫外辐射；氧化损伤
中图分类号：R285                             文献标志码：ADOI：10.3969/j.issn.1007-7146.2022.01.009

Abstract: In this paper, the results showed that single carotenoid and their combinations significantly reduced LDH activity and MDA content (P<0.05), and had protective effects on UVA-induced oxidative damage to HSF cells. At the same concentration, astaxanthin had better LDH reduction activity than β-carotene, and had better protective effect on oxidative damage of HSF cells. This study provides a theoretical basis for the development of cosmetics and health care products with the protective effect on UVA radiation oxidative damage with carotenoids.
Key words: astaxanthin; β-carotene; echinenoneon; ultraviolet radiation; oxidative damage
（Acta Laser Biology Sinica, 2022, 31(1): 061-069）