摘　要：叶绿素酶（CLH）是植物叶绿素降解过程中的关键酶，在植物生长发育过程中发挥着重要作用。为了解小麦CLH基因家族成员在叶绿素降解过程中的功能差异，本试验采用生物信息学分析方法和实时荧光定量PCR（qRT-PCR）技术对小麦CLH基因家族进行鉴定和初步功能分析，结果表明：小麦中共鉴定到13个CLH成员（TaCLH1～TaCLH13），分布在3A、3B、3D、4B、5A、5D、6A、6B和6D染色体上；其中13个家族成员的启动子中均含有植物激素响应元件、环境胁迫响应元件和植物生长发育响应元件；分析TaCLHs在不同组织和不同发育阶段中的表达模式发现，大部分成员在叶/嫩枝和穗中有表达；结合qRT-PCR分析发现，TaCLHs在不同的生长发育阶段参与叶绿素降解过程。这些研究结果都为后续研究相关基因的功能奠定了基础。
关键词：小麦；CLH基因家族；叶绿素降解；表达分析；qRT-PCR
中图分类号：S512.1；Q78                     文献标志码：ADOI：10.3969/j.issn.1007-7146.2022.01.008

Abstract: Chlorophyllase is the key enzyme in plant chlorophyll degradation process and plays an important role in plant growth and development. To understand the functional differences of CLH gene family members in wheat during chlorophyll degradation, the bioinformatics analysis method and real-time fluorescence quantitative (qRT-PCR) technology were used to identify and analyze the CLH gene family in wheat. The results showed that 13 TaCLHs (TaCLH1 ～ TaCLH13) were identified in wheat genome and distributed on chromosomes 3A, 3B, 3D, 4B, 5A, 5D, 6A, 6B and 6D. The promoter regions of TaCLHs contained plant hormone response elements, environmental stress response elements and plant growth response elements. Analysis of TaCLHs expression patterns in different tissues and different developmental stages showed that most of the members expressed in leaves/shoots and panicles. In addition, qRT-PCR analysis showed that TaCLHs were involved in chlorophyll degradation at different growth stages, which laid the foundation for further study of CLH gene function in wheat.
Key words: wheat; CLH gene family; chlorophyll degradation; expression analysis; qRT-PCR
（Acta Laser Biology Sinica, 2022, 31(1): 050-060）