摘　要：斑马鱼广泛地应用于骨退化相关的机制研究和新药开发过程中。目前临床应用检测骨密度（BMD）的方法主要有单光子吸收、双光子吸收、双能X线吸收、定量CT和超声技术等，上述方法均难用于斑马鱼的研究中。本文旨在寻求一种新式的、适用性强和操作简易的定量分析斑马鱼BMD的方法。对采集到的使用125 μmol/L泼尼松龙溶液诱导骨质疏松的斑马鱼在0、7、14、21 d的光学相干层析成像的图像依次进行灰度化、二值化、图像分割、阈值分割和密度投影，最后进行了颅骨BMD统计。试验结果表明，此方法可以有效地定量分析出不同天数斑马鱼骨质疏松程度，适用于研究泼尼松龙诱导的不同天数对斑马鱼BMD的影响、大通量斑马鱼骨质疏松药物筛选、药物评价等，是一种将斑马鱼颅骨BMD定量化分析的新方法。
关键词：斑马鱼；颅骨；光学相干层析术；骨密度；骨退化
中图分类号：R-332                          文献标志码：ADOI：10.3969/j.issn.1007-7146.2022.01.006

Abstract: Zebrafish are widely used in mechanistic studies related to bone degradation and during new drug development. At present, the clinical application methods of bone mineral density (BMD) mainly include single-photon absorption, two-photon absorption, dual-energy X-ray absorption, quantitative CT, and ultrasonic technology. All these methods are difficult to use in zebrafish. This article aims to seek a new, applicable and easy-to-operate method for quantitative analysis of zebrafish bone mineral density. The optical coherence tomography images of zebrafish with 125 μmol/L prednisolone solution induced osteoporosis at 0, 7, 14, and 21 days were sequentially grayed, binarized, image segmented, and threshold segmented, and were done density projection, and finally bone density statistics. The experimental results showed that this method can effectively and quantitatively analyze the degree of osteoporosis in zebrafish on different days. This method is suitable for studying the effects of different exercise modes on zebrafish bone mineral density during the growth period, high-throughput zebrafish osteoporosis drug screening, drug evaluation, etc. It is a new method for quantitative analysis of zebrafish bone mineral density.
Key words: zebrafish; skull; optical coherence tomography; bone mineral density; bone degeneration
（Acta Laser Biology Sinica, 2022, 31(1): 036-042）