摘　要：激光拉曼光谱是一种可以作为诊断组织分子结构的物理探针，具有辅助诊断良恶性肿瘤和判断预后的作用。本研究探索应用激光共聚焦拉曼光谱对脑胶质瘤进行快速诊断与分级，辅助用于术中及术后胶质瘤诊断与鉴别诊断。本研究使用Renishaw InVia激光共聚焦拉曼光谱仪，采用514 nm激发光源，测量新鲜Ⅲ、Ⅳ级人脑胶质瘤组织及正常人脑组织样本共计14例。结果显示：采用绝对峰位移比较、特征峰位移比值、聚类分析等方法分析激光共聚焦拉曼光谱检测数据，证实正常脑组织与脑胶质瘤组织在1 664、2 852、2 882和2 930 cm–1处表现出明显的拉曼位移差异；Ⅲ级、Ⅳ级脑胶质瘤组织之间亦在1 235、1 260、1 311、1 360、1 583、1 660、3 160和3 300 cm–1处表现出明显的拉曼绝对位移差异，并在I1 660/I1 583、I1 311/I1 360、I1 235/I1 360等处表现出显著的、具有诊断意义的相对位移差异。综上，利用激光共聚焦拉曼光谱能区分脑胶质瘤与正常脑组织，鉴别诊断Ⅲ、Ⅳ级脑胶质瘤分级准确率可达86%。因此，激光共聚焦拉曼光谱是一种可行、准确的对脑胶质瘤进行诊断与分级的辅助手段。
关键词：激光共聚焦拉曼光谱；Ⅲ/Ⅳ级脑胶质瘤；拉曼位移差异；诊断；分级
中图分类号：R739.41                         文献标志码：A          DOI：10.3969/j.issn.1007-7146.2021.06.010

Abstract: Laser Raman spectroscopy is a physical probe that can be used to diagnose the molecular structure of tissues. In this study, Renishaw InVia laser confocal Raman spectrometer and 514 nm laser was used. A total of 14 cases of fresh grade Ⅲ and Ⅳ human brain glioma tissues and normal human brain tissue samples were measured. The results indicated that significant Raman peak-to-peak displacement at 1 664, 2 852, 2 882 and 2 930 cm–1 peaks were found between normal brain tissue and glioma lesion tissue. Furthermore, the difference in Raman peak-to-peak displacement between grade Ⅲ and grade Ⅳ glioma tissues were at 1 235, 1 260, 1 311, 1 360, 1 583, 1 660, 3 160 and 3 300 cm–1 peaks. Meanwhile, the differences of relative displacement at I1 660/I1 583、I1 311/I1 360、I1 235/I1 360 were obvious and showed diagnostic sense. In conclusion, Raman spectroscopy could distinguish brain tissues among normal brain, grade Ⅲ glioma and grade Ⅳ glioma, and the accuracy could be up to 86%. Thus, Raman spectroscopy could be used as an accurate and rapid method for glioma diagnosis and grading.
Key words: Raman spectroscopy; grade Ⅲ & Ⅳ glioma; Raman shift difference; diagnosis; grade
（Acta Laser Biology Sinica, 2021, 30(6): 553-558）