摘　要：为了研究血卟啉单甲醚（HMME）介导的光动力抗微生物化疗法（PACT）对牙龈卟啉单胞菌（Porphyromonas gingivalis）的杀菌效果，采用荧光分光光度计观察HMME光照后产生活性氧的荧光量，后采用平板菌落计数法检测HMME介导的PACT对牙龈卟啉单胞菌的体外杀菌效果，并用激光共聚焦显微镜（CLSM）观察各组处理后死活菌的分布情况，使用电泳光散射法检测HMME与牙龈卟啉单胞菌的zeta电位。结果表明：HMME在光照下主要产生单线态氧（P<0.05），其介导的PACT组细菌存活率明显下降，与对照组相比有统计学差异（P<0.05）；CLSM观测PACT组发现大量红色荧光，说明HMME介导PACT可有效杀灭牙龈卟啉单胞菌，HMME与牙龈卟啉单胞菌均为负电位，影响二者结合。HMME介导的PACT可有效杀灭牙龈卟啉单胞菌，为PACT应用于临床治疗牙周炎提供了试验依据，也表明后期需要研发带正电荷的新型光敏剂，以提高光动力杀菌效果。
关键词：牙龈卟啉单胞菌；血卟啉单甲醚；光动力抗微生物化疗法；牙周炎；激光
中图分类号：Q935　         　  　　文献标志码：A                    DOI：10.3969/j.issn.1007-7146.2021.06.009

Abstract: The present study aimed to investigate the effect of photodynamic antimicrobial chemotherapy (PACT) mediated by hemoporphyrin monomethyl ether (HMME) on Porphyromonas gingivalis. Fluorescence spectrophotometer was used to observe the fluorescence of reactive oxygen species (ROS) produced by HMME after illumination, a colony-counting method and confocal laser scanning microscopy (CLSM) were used to observe the neutralization of P. gingivalis, HMME and P. gingivalis zeta potentials were detected by electrophoresis light scattering method. Results showed that under light, HMME mainly produced singlet oxygen (P<0.05). The survival rate of HMME-PACT group was significantly lower than the control group (P<0.05). A large amount of red fluorescence was found in PACT group observed by CLSM, indicating that HMME-PACT could effectively kill P. gingivalis. Both HMME and P. gingivalis are negative potential, which affects their combination. HMME-PACT can effectively kill P. gingivalis, which provides experimental basis for the application of PACT in clinical treatment of periodontal disease, and also indicates that it is necessary to develop new photosensitizers with positive charge in the later stage to improve the photodynamic bactericidal effect.
Key words: porphyromonas gingivalis; hematoporphyrin monomethylether; photodynamic antimicrobial chemotherapy; periodontitis; laser
（Acta Laser Biology Sinica, 2021, 30(6): 548-552）