摘　要：人巨细胞病毒（HCMV）感染是常见的病毒性疾病，目前无有效的疫苗，故抗病毒药物筛选对HCMV的治疗具有重要意义。为了构建一种抗巨细胞病毒体外药物快速筛选模型，本研究以带有荧光素酶标签的鼠巨细胞病毒细菌人工染色体（Luc-MCMV-BAC）为基础，将其转染至小鼠成纤维细胞NIH-3T3，成功获得重组病毒Luc-MCMV，且其能够在细胞中稳定发光。噬斑法测定的病毒生长曲线显示，Luc-MCMV与野生型病毒Wt-MCMV具有相似的生长动力学，说明Luc-MCMV 能够反映Wt-MCMV的生长状况，可用于后续抗病毒药物的筛选评价。进一步选用更昔洛韦（Ganciclovir）作为阳性对照药物，应用该体系对6种中药单体进行了筛选，结果显示黄芩素和黄芪甲苷具有抗MCMV活性，为抗HCMV药物的筛选奠定了前期基础。
关键词：巨细胞病毒；重组病毒；抗病毒药物筛选；荧光素酶；筛选模型
中图分类号：R392.9                           文献标志码：A                      DOI：10.3969/j.issn.1007-7146.2021.06.006

Abstract: Human cytomegalovirus (HCMV) infection is a common viral disease. In view of the lack of effective vaccines against HCMV currently, the screening of antiviral drugs for treatment of HCMV are of great significance. In order to construct a rapid screening model of anti-cytomegalovirus drugs in vitro, in this study, a murine cytomegalovirus bacterial artificial chromosome with a luciferase tag (Luc-MCMV-BAC) was transfected into mouse fibroblast NIH-3T3 cells. The recombinant virus Luc-MCMV was successfully obtained, and it can stably emit light in cultured cells. The virus growth curves determined by the plaque method showed that Luc-MCMV displayed similar growth kinetics comparable to those of wild-type virus Wt-MCMV, indicating that Luc-MCMV can be used to screen drugs and evaluate the antiviral activity. Furthermore, using Ganciclovir as a positive control drug, six kinds of traditional Chinese medicine monomers were screened using this system. Results showed that Baicalein and Astragaloside IV had anti-MCMV activity, which laid the foundation for the screening of anti-HCMV drugs.
Key words: cytomegalovirus; recombinant virus; antiviral drug screening; luciferase; screening model
（Acta Laser Biology Sinica, 2021, 30(6): 526-532）