摘　要：视网膜母细胞瘤（RB）严重损害儿童视力健康与生命安全。本研究建立了一种光学相干层析成像（OCT）辅助的RB斑马鱼模型构建的新方法，并利用OCT对RB细胞在眼内的分布与存活状态进行活体评估。试验随机选取了24条正常斑马鱼作为研究对象，其中21条鱼用于构建RB斑马鱼模型，具体做法是在OCT实时成像下，通过针剂注射带荧光的RB细胞到其玻璃体内部，剩余3条鱼作为对照。细胞注射后的0、24、48、72 h观察RB细胞的分布与存活状态，同时，建模前后都对斑马鱼的眼睛进行体视荧光显微镜（SFM）成像，以作为对照。结果表明，OCT可高分辨率且大深度地显示斑马鱼玻璃体内RB细胞的三维分布，OCT图像分析可以定量说明RB细胞的数量变化情况，且OCT结果与SFM结果高度匹配。综上所述，斑马鱼模型与OCT成像技术结合的策略有望为RB疾病研究提供新的方法和装备。
关键词：视网膜母细胞瘤；斑马鱼；光学相干层析成像；玻璃体；患儿
中图分类号：R-332                             文献标志码：A              DOI：10.3969/j.issn.1007-7146.2021.06.005

Abstract: Retinoblastoma (RB) seriously damages children’s vision health and life safety. This study established a new method of optical coherence tomography (OCT) assisted RB zebrafish model construction, and used OCT to evaluate the distribution and survival status of RB cells in the eyes in vivo. Twenty-four normal zebrafish were used in this study, of which 21 were used to construct the RB zebrafish model and the remaining 3 were used as control. The specific method was to inject fluorescent RB cell into the eyeball through an injection under OCT real-time imaging. The distribution and survival status of cell were observed at the 0th, 24th, 48th, and 72th hour after cell injection. At the same time, the zebrafish eyes were imaged with a stereo fluorescent microscope (SFM) before and after modeling, as a control. The results showed that high-resolution, large-depth three-dimensional distribution of RB cell clusters in the eyes could be obtained by OCT. Further OCT image intensity analysis could quantify the number of RB cells, and the results were highly matched with the results of the SFM images. In conclusion, the strategy of combining zebrafish model with OCT imaging technology is expected to provide new methods and equipment for the study of RB.
Key words: retinoblastoma; zebrafish; optical coherence tomography; vitreous; sick child
（Acta Laser Biology Sinica, 2021, 30(6): 518-525）