摘　要：黑腹果蝇（Drosophila melanogaster）的心脏发育调控基因与人类的相关基因具有高度同源性。Lbe基因是果蝇心脏发育的重要调控基因。为了深入研究Lbe基因在心脏发育中的调控功能，采用DNA免疫技术制备了抗果蝇Lbe蛋白的多克隆抗体。通过PCR扩增Lbe基因序列，将该序列与真核表达载体pCAGGS-P7同源重组，以该同源重组载体免疫4周龄小鼠，获得含有抗Lbe多克隆抗体的血清。蛋白质印迹（Western blot）和胚胎抗体染色结果表明，该抗体的特异性较好。该抗体为随后的Lbe功能的研究奠定了基础。
关键词：果蝇；心脏发育；Lbe；DNA免疫；多克隆抗体
中图分类号：Q78　　　　　　　文献标志码：ADOI：10.3969/j.issn.1007-7146.2021.04.006

Abstract: The heart development genes of Drosophila melanogaster are highly conserved with human heart development genes. Lbe (Ladybird early) is one of the important genes involved in Drosophila heart development. In order to further understand the regulatory role of Lbe gene in heart development, the anti-Lbe antibody is prepared with DNA immunization technique. The Lbe coding sequence was amplified with PCR, and then it was homologously recombined with the eukaryotic expression vector pCAGGS-P7. This homologous recombinant vector was used as antigen to immunize four weeks old mice to obtain the serum containing anti-Lbe polyclonal antibody. Western blot and embryo antibody staining results showed that the specificity of the antibody was good. The antibody provides a foundation for the subsequent study of function of Lbe.
Key words: Drosophila; heart development; Ladybird early; DNA immunization; polyclonal antibody
（Acta Laser Biology Sinica, 2021, 30(4): 333-338）