摘　要：为探究egl-9家族缺氧诱导因子1（EGLN1）在肾透明细胞癌（ccRCC）中的表达情况及临床意义，基于TCGA数据库中ccRCC患者的基因表达数据分析EGLN1在ccRCC组织和正常肾组织中的差异表达情况，应用UALCAN平台分析ccRCC中EGLN1在不同角度下的表达差异。基于TCGA数据库中ccRCC的临床数据，对EGLN1高低表达组进行生存分析，对EGLN1表达量与总生存期的相关性进行Cox分析，同时对临床数据中EGLN1基因表达情况进行基因富集分析。通过STRING数据库检索了编码蛋白间可能的潜在相互作用，并构建了蛋白质互作网络将其表示出来。获取EGLN1相关蛋白，并通过GEPIA平台进行相关分析。分析TCGA数据库中样本表达数据可知，EGLN1在ccRCC中的表达高于正常肾组织。应用UALCAN平台分析发现，EGLN1的高表达与患者的年龄有一定的相关性。生存分析显示EGLN1高表达时，ccRCC患者预后较好。单因素及多因素Cox分析提示EGLN1的表达量是ccRCC患者生存期的独立风险因素。基因富集分析提示EGLN1基因的表达与肾细胞癌（RCC）、癌症途径、泛素介导蛋白水解途径、细胞凋亡通路等密切相关。结果显示，EGLN1可能成为评估ccRCC预后的分子标志。
关键词：EGLN1；肾透明细胞癌；癌症基因组图谱；生物信息学；Cox分析
中图分类号：R692.6　　　　　　文献标志码：ADOI：10.3969/j.issn.1007-7146.2021.02.013

Abstract: In order to explore the expression and clinical significance of egl-9 family hypoxia-inducible factor 1 (EGLN1) in clear cell renal cell carcinoma (ccRCC), based on the gene expression data of ccRCC patients in the TCGA database to analyze EGLN1 in ccRCC tissues and normal kidney tissues, the difference in expression of EGLN1 in ccRCC was analyzed using the UALCAN platform. Based on the ccRCC clinical data in the TCGA database, the survival analysis of the EGLN1 high and low expression group, the Cox analysis of the correlation between EGLN1 expression and overall survival, and the gene enrichment analysis of the EGLN1 gene expression in the clinical data were performed. The possible potential interactions between encoded proteins were searched through the STRING database, and a protein interaction network was constructed to express them, obtain EGLN1 related proteins, and perform related analysis on the GEPIA platform. Analysis of the sample expression data in the TCGA database shows that the expression of EGLN1 in ccRCC is higher than that in normal kidney tissue. The analysis of the UALCAN platform found that the high expression of EGLN1 is correlated with the age of the patient. Survival analysis showed that ccRCC patients had a better prognosis when EGLN1 was overexpressed. Univariate and multivariate Cox analysis indicated that the expression of EGLN1 is an independent risk factor for the survival of ccRCC patients. Gene enrichment analysis suggests that the expression of EGLN1 gene is closely related to RCC pathway, ubiquitin-mediated proteolysis pathway, and apoptosis pathway. The results show that EGLN1 may be a molecular marker for evaluating the prognosis of ccRCC.
Key words: EGLN1; clear cell renal cell carcinoma; cancer genome atlas; bioinformatics; Cox analysis
（Acta Laser Biology Sinica, 2021, 30(2): 185-192）