摘　要：为了探究中草药添加剂对罗氏沼虾（Macrobrachium rosenbergii）免疫功能的影响，以罗氏沼虾为对象，在水温 20～30℃、pH 7～8的试验条件下，往饲料中分别添加质量分数为0%、1.0%、1.5%、2.0%、2.5%、3.0%的复方中草药制剂，连续饲喂25 d。结果显示：随着饲料中复方中草药质量分数的提高，罗氏沼虾的生长指标、血清中碱性磷酸酶（AKP）活性、血清蛋白含量均呈现先上升后下降的趋势，试验组相较于对照组有很大提高；中草药质量分数的提高引起的血清中蛋白含量的提高远远大于肌肉中蛋白的含量，在各试验组中2.5%的复合中草药制剂的添加量效果最好。试验证明，添加复方中草药制剂对罗氏沼虾的免疫活性、生长性能均有明显的促进作用。该结果能够为罗氏沼虾的健康养殖提供基础资料。
关键词：罗氏沼虾；复方中草药；AKP活性；生长性能；蛋白含量
中图分类号：S966.12　　　　　　文献标志码：A DOI：10.3969/j.issn.1007-7146.2021.02.008

Abstract: To explore the effect of Chinese herbal medicine additive on the immune function of the Macrobrachium rosenbergii, this experiment took Macrobrachium rosenbergii as an object, adding 0%, 1.0%, 1.5%, 2.0%, 2.5%, 3.0% compound Chinese herbal additive into the feed, fed for 25 d, under the water temperature 20～30℃ and pH 7～8. The results showed that as the increase of the concentration of compound Chinese herbal medicine additive in the feed, the growth index, the activity of AKP in serum and the content of the serum protein of Macrobrachium rosenbergii showed a tendency to go up first and then down. The experiment group had greatly improvement compared with the control group. The concentration of Chinese herbal medicine increased the protein content in serum far more than the protein content in muscle did. In each experimental group, the additive amount of 2.5% had the best effect. The experiment showed that adding compound Chinese herbal medicine additive has obvious effects on immune activity and growth performance of Macrobrachium rosenbergii, which can provide basic information for healthy aquaculture of Macrobrachium rosenbergii.
Key words: Macrobrachium rosenbergiias; the compound Chinese herbal medicine; AKP activity; growth performance; protein content
（Acta Laser Biology Sinica, 2021, 30(2): 147-153）