摘　要：为了探究聚乙二醇化黑色TiO2介导的光动力疗法体外灭活HL60细胞的潜力，先后采用水热还原法和表面修饰法制备不同质量比的聚乙二醇化黑色TiO2（PEG@B-TiO2）纳米复合材料。通过X射线衍射（XRD）、傅里叶红外光谱（FTIR）、紫外可见吸收光谱（UV-VIS）、荧光光谱（FS）、透射电子显微镜（TEM）及粒度仪研究材料的理化性质。用活性氧检测试剂盒检测材料的活性氧（ROS）产量。通过细胞增殖试验（CCK-8法）比较不同纳米颗粒对HL60细胞的暗毒性和可见光下的光毒性，探究最佳材料。结果表明：合成的PEG@B-TiO2分散性较好，结晶度较高，可见光吸收能力强，电子空穴复合率低；同时，暗毒性试验证明了药物的生物相容性好，与纯TiO2相比，PEG@B-TiO2在光照下对HL60细胞的灭活效率更高。这表明其有望成为光动力治疗白血病的有效光敏剂。
关键词：光动力疗法；光敏剂；可见光；活性氧；HL60细胞
中图分类号：R454.2　　　　　　  文献标志码：ADOI：10.3969/j.issn.1007-7146.2021.02.005

Abstract: In order to explore the potential of pegylated black TiO2 mediated photodynamic therapy to inactivate HL60 cells, black pegylated TiO2 (PEG@B-TiO2) nanocomposites with different mass ratios were synthesized by the combination of hydrothermal reduction and surface modification method. The physicochemical properties of this prepared samples were characterized by X-ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR), ultraviolet and visible spectrum (UV-VIS), fluorescence spectra (FS), transmission electron microscopy (TEM), and granulometer, respectively. Then reactive oxygen species detection kit was used to detect the yield of reactive oxygen (ROS). In order to determine the drug which is most effective, cell proliferation experiment (CCK-8 method) was conducted to compare the toxity of different drugs on HL60 cells in darkroom condition and under visible light irradiation. The results indicated the prepared nanomaterials were with good dispersion, high crystallinity, low electron hole pair recombination rate and strong absorbability to visible light. At the same time, dark toxicity experiment suggested that the biocompatibility was good. Compared with pure TiO2, PEG@B-TiO2 showed higher inactivation efficiency under light irradiation, which manifested that they were expected to be an effective photosensitizer for treating leukemia by photodynamic therapy.
Key words: photodynamic therapy; photosensitizer; visible light; reactive oxygen; HL60 cells
（Acta Laser Biology Sinica, 2021, 30(2): 123-130）