摘　要：以野生型拟南芥（Col-0）与脂质转运蛋白（LTP2）突变体ltp2为试验材料，初步研究了LTP2对乙烯信号转导的影响。采用乙烯前体1- 氨基环丙烷羧酸（ACC）处理野生型与突变体材料，结果表明，LTP2基因的表达受乙烯诱导，“三重反应”的表型显示突变体ltp2对乙烯的敏感性弱于野生型。采用荧光定量PCR（Real-time PCR）进一步分析了LTP2基因的功能缺失突变对乙烯信号转导相关基因（RTE1、ETR1、CTR1、EIN2和EIN3）、乙烯响应因子基因（ERF1、ERF2和ERF9）以及乙烯相关蜡质合成酶基因（CER3和CER6）表达的影响。无ACC处理时，ltp2突变体中ETR1、EIN2、ERF1、ERF2、ERF9和CER6等基因表达量均高于野生型，而EIN3和CER3基因的表达量则略低于其野生型。在ACC处理后，ltp2突变体中RTE1表达量仅为野生型的一半左右，CTR1与CER3的表达量高于野生型，3种乙烯响应因子基因（ERF1、ERF2和 ERF9）的表达量均低于野生型。以上结果表明，LTP2参与了乙烯信号转导的调控。
关键词：拟南芥；ltp2突变体；乙烯；三重反应；信号转导
中图分类号：Q945.4　　　　　　  文献标志码：ADOI：10.3969/j.issn.1007-7146.2021.01.011

Abstract: The Arabidopsis thaliana wild type (Col-0) and lipid transfer protein 2 (LTP2) mutant ltp2 were used as experimental materials to study the roles of LTP2 in ethylene signal transduction. Using ACC, which is a precursor of ethylene to treat wild type and ltp2 mutant, the results showed that the expression of LTP2 was induced by ethylene, and the phenotypes of triple response also showed that the sensitivety of ltp2 was weaker than that of wild type. Furthermore, we analyzed the effects of loss-of-function mutation in the LTP2 gene to some genes by Real-time PCR, including the relative expression of ethylene signal transduction genes (RTE1, ETR1, CTR1, EIN2 and EIN3), ethylene response factor genes (ERF1, ERF2 and ERF9) and waxy synthetase genes (CER3 and CER6). In the absence of ACC treatment, the relative expression levels of ETR1, EIN2, ERF1, ERF2, ERF9 and CER6 of ltp2 were higher than that of the wild type, while EIN3 and CER3 of ltp2 were lower than that of the wild type. After application of ACC treatment, the relative expression level of RTE1 in the ltp2 mutant was about half that of the wild type, and the relative expression levels of CTR1 and CER3 were higher than that of the wild type, and all the examined three ERF genes(ERF1, ERF2, ERF9) showed higher expression levels compared to the wild type. The above results suggest that LTP2 is involved in ethylene signal transduction.
Keywords: Arabidopsis thaliana; ltp2 mutant; ethylene; triple response; signal transduction
（Acta Laser Biology Sinica, 2021, 30(1): 084-090）