摘  要：通过化学气相沉积法制备了g-C3N4@C-TiO2纳米颗粒，利用X射线衍射（XRD）、荧光光谱（FS）、透射电子显微镜（TEM）、紫外-可见吸收光谱（UV-Vis）、能谱（EDS）等对纳米颗粒进行表征。分别研究了暗室条件及光照条件下g-C3N4@C-TiO2纳米颗粒对HL60细胞的作用效果。采用CCK-8法探究了一系列质量浓度梯度的纳米颗粒处理HL60细胞后的存活率，并且通过荧光探针标记技术检测细胞内活性氧水平。试验结果表明，在C-TiO2表面包裹g-C3N4，可将TiO2可吸收的光波长范围拓展至可见光波段。制备的g-C3N4@C-TiO2粒径在10～20 nm，满足其进入细胞的尺寸要求。暗毒性试验表明g-C3N4@C-TiO2纳米颗粒在暗室条件下对细胞的毒性较小，证明了其具有良好的生物相容性。同时，与TiO2和C-TiO2纳米颗粒处理组相比，g-C3N4@C-TiO2处理组的光动力灭活效率高达（76.5±1.9）%，表明其可能成为治疗白血病的潜在光敏剂。
关键词：光动力治疗；g-C3N4@C-TiO2；光敏剂；HL60细胞；肿瘤细胞凋亡
中图分类号：R454.2　　　　　　  文献标志码：ADOI：10.3969/j.issn.1007-7146.2021.01.006

Abstract: C-doped TiO2 nanoparticles combined with g-C3N4 were prepared by chemical vapor deposition, using X-ray diffraction, fluorescence spectroscopy, transmission electron microscopy, UV-Vis absorption spectroscopy and energy dispersive spectrometer to characterize nanoparticles. The effects of g-C3N4@C-TiO2 nanoparticles on HL60 cells in vitro were explored in dark and under light irradiation. Cell Counting Kit-8 (CCK-8) method was used to investigate the viability of HL60 cells treated with nanoparticles with a series of concentration gradients, and the level of intracellular reactive oxygen species was detected via fluorescent probe labeling technology. The experimental results showed that by modifying g-C3N4 on the surface of C-TiO2, the absorption wavelength range of TiO2 could be extended to the visible band. The particle size of g-C3N4@C-TiO2 prepared was about 10～20 nm, which met the size requirement of entering cells. Dark toxicity experiment proved that g-C3N4@C-TiO2 nanoparticles were less toxic to cells under darkroom conditions, which suggested they had good biocompatibility. Meanwhile, compared with the groups treated with TiO2 and C-TiO2 nanoparticles, the photocatalytic inactivation efficiency of the group treated with g-C3N4@C-TiO2 was as high as (76.5±1.9)% under light irradiation, indicating its potential as a potential photosensitizer for the treatment of leukemia.
Key words: photodynamic therapy; g-C3N4@C-TiO2; photosensitizer; HL60 cells; apoptosis of tumor cells
（Acta Laser Biology Sinica, 2021, 30(1): 043-051）