摘　要：本试验通过二次谐波和偏振光影像技术检测烧烫伤小鼠瘢痕胶原纤维排列和密度的变化。在小鼠背部造烧烫伤深度模型，模仿临床烧烫伤处理方法去除结痂，6个月时通过二次谐波和液晶偏振光影像技术从活体皮肤水平面和离体组织矢状面检测烧烫伤小鼠瘢痕和瘢痕周围乳头层以及网状层胶原纤维排列和密度的变化。二次谐波成像结果显示，正常皮肤乳头层和网状层胶原纤维呈随机排列的网状结构，排列一致性低，胶原纤维排列稀疏，面密度少，而瘢痕和瘢痕周围乳头层以及网状层胶原纤维排列一致性和面密度均明显增加。液晶偏振光影像技术结果表明，与正常皮肤比较，瘢痕和瘢痕周围乳头层以及网状层胶原纤维方位角变异程度明显降低，相位差值增加。本试验通过二次谐波和偏振光影像技术发现，瘢痕和瘢痕周围乳头层以及网状层胶原纤维的排列和密度均发生了改变，而且二次谐波与液晶偏振光影像技术检测的结果趋势相同，这为将来仅通过二次谐波成像技术无创鉴别瘢痕组织奠定了基础，同时为在人体上诊断瘢痕和评估瘢痕治疗效果提供了方法。
关键词：二次谐波成像技术；偏振光影像技术；烧烫伤；瘢痕；胶原纤维

Abstract: Arrangement and density of collagen fibers in burn scar were detected by second harmonic and polarized light imaging techniques in mice. The burn model was established on the back of mice, excisions of burn wounds were undertaken following current clinical practice, arrangement and density of collagen fibers in papillary layer and reticular layer of scar and scar edge were studied by second harmonic and polarized light imaging techniques in vivo and ex vivo. The results of second harmonic imaging showed that collagen fibers were randomly arranged, arrangement consistency was low, collagen fibers were sparse, area density was small in papillary layer and reticular layer of normal skin, however, arrangement consistency and area density were increased significantly in papillary layer and reticular layer of scar and scar edge. The results of liquid crystal polarized light imaging technology showed that variation of azimuth of collagen fibers were reduced significantly, retardance were increased in papillary layer and reticular layer of scar and scar edge compared with normal skin. We found that arrangement and density of collagen fibers in papillary layer and reticular layer of scar and scar edge were changed by second harmonic and polarized light imaging technology, the results of second harmonic and liquid crystal polarized light imaging technology had the same trend. The results would lay the foundation for non-invasive identification of scar tissue through second harmonic imaging technology in future, and provide methods for diagnosing scars in human and assess effectiveness of scar treatment.
Key words: second harmonic imaging; polarized light imaging; burn; scar; collagen fibers