复合微生物菌剂在长毛对虾标苗养殖过程中的应用研究

谢芝玲,徐佳莹,潘 登,刘 峰,陈 燕,杨焕胜,印遇龙,夏立秋,张友明,涂 强

激光生物学报 ›› 2020, Vol. 29 ›› Issue (3) : 225-230.

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激光生物学报 ›› 2020, Vol. 29 ›› Issue (3) : 225-230.
研究论文

复合微生物菌剂在长毛对虾标苗养殖过程中的应用研究

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Application of Complex Microbial Preparations in Penaeus penicillatus Breeding

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摘要

复合微生物菌剂是一类有效的生物防治剂,在农业和水产养殖业中都起着重要的作用。本研究以长毛对虾(Penaeus penicillatus)标苗为试验对象,共分为4个组别,组1投喂无微生物菌剂添加的饲料作为对照组,组2、组3和组4为试验组,投喂有复合微生物菌剂添加的饲料,其中微生物菌剂分别占饲料质量的1.0%、1.5%和2.0%。每个组进行33 d 的养殖,测定养殖期间对虾生长性能、养殖水池底泥和水质相关性质,探讨微生物菌剂对对虾生长的影响。结果显示:试验前对照组与3个试验组的体重无显著差异(P>0.050),试验后各试验组的体重均高于对照组(P<0.050),其中组4体重达(0.60±0.03)g,表明微生物菌剂的使用促进了对虾标苗的生长;水体弧菌个数在试验不同时间之间有显著差异(P=0.000),对照组呈上升趋势,第30天时未添加微生物菌剂的对照组弧菌数目最高,表明微生物菌剂能控制水体有害菌的生长;水体中的溶解氧(DO)含量在养殖不同时间之间均具有显著差异(F=191.959,P=0.000),NH4-N、NO2-N和NO3-的含量均是如此,F值分别为250.633、659.806和1 937.649,P值均为0.000,从养殖第10天开始,试验组DO含量均高于对照组,而试验组的NH4-N、NO2-N和NO3-N含量均低于对照组。综上所述,复合微生物菌剂对对虾有明显地促生长作用,并且还能在一定程度上净化养殖环境。

Abstract

Complex microbial preparations are a class of effective biological control agents that play an important role in agriculture and aquaculture. In this study, the Penaeus penicillatus was used as the test object. Four microbial preparation treatments were arranged (Group 1~4) with the microbial agents accounted for 0.0%, 1.0%, 1.5%and 2.0%of feed quality, respectively. Group 1 was set to be the control without any microbial preparations insertion. The whole experiment lasted 33 days. To investigate the effect of microbial agents on the growth of prawn, several data had been measured, such as the growth performance of prawn, the properties of the bottom mud and water quality. The results showed that there were no significant differences in the body weight of the four groups before experiment (P>0.050). After the experiment, the body weight of each experimental group was higher than that of the control group (P<0.050). Above all, the body weight of group 4 was(0.60±0.03)g, indicating that the use of complex microbial preparations promoted the growth of prawn. Meanwhile, there were also significant differences in the number of Vibrios in water between different times of the experiment (P=0.000). The number of Vibrios in the control group displayed an upward trend. On the 30th day, the number of Vibrios in the control group without microbial agents was the highest, indicating that microbial agents could control the growth of harmful bacteria in aquaculture water environment. The DO value in the water was significantly different in different culture time (F=191.959, P=0.000). The content of NH4-N、NO2-N and NO3-N was similar to the above. The F value was 250.633,659.806 and 1 937.649, respectively, whereas Pvalue was 0.000. After the 10th day of culture, the DO content in the experimental group was higher than that in the control group, whereas the NH4-N、NO2-Nand NO3-N value in the experimental group were lower than that in the control group. In summary, the compound microbial bacteria agent can obviously promote the growth of prawn and purify the breeding environment to a certain extent.

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谢芝玲,徐佳莹,潘 登,刘 峰,陈 燕,杨焕胜,印遇龙,夏立秋,张友明,涂 强. 复合微生物菌剂在长毛对虾标苗养殖过程中的应用研究[J]. 激光生物学报. 2020, 29(3): 225-230
Application of Complex Microbial Preparations in Penaeus penicillatus Breeding[J]. Acta Laser Biology Sinica. 2020, 29(3): 225-230

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