荧光竞争结合技术在昆虫嗅觉相关蛋白结合能力检测上的应用

彭 竹,黄 丽,赵慧婷

激光生物学报 ›› 2020, Vol. 29 ›› Issue (2) : 106-112.

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激光生物学报 ›› 2020, Vol. 29 ›› Issue (2) : 106-112.
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荧光竞争结合技术在昆虫嗅觉相关蛋白结合能力检测上的应用

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Application of  Fluorescence Competitive Binding Technology in Detection  of the Binding Ability of Insects Olfactory Related Proteins

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摘要

昆虫嗅觉相关蛋白在其觅食、交配、防御、产卵、信息交流、栖息地选择等行为及生理活动方面起着十分重要的作用。荧光竞争结合试验是目前用于检测昆虫气味结合蛋白(OBPs)和化学感受蛋白(CSPs)与外界环境气味分子结合特性的典型方法。本文对荧光竞争结合试验在昆虫OBPs和CSPs上的相关研究及应用进行综述,为深入了解昆虫嗅觉识别机制、制定害虫防治和经济昆虫保护策略提供理论参考。

Abstract

Olfactory related proteins of insects play an important role in their behaviors and physiological activities such as foraging, mating, defense, spawning, information exchange and habitat selection Fluorescence competitive binding assay is a typical method for detecting the binding properties of insect OBPs and CSPs with environmental odor molecules In this paper, some researches and applications  on fluorescence competitive binding assay used for insects olfactory proteins (OBPs and CSPs) are reviewed It provides  theoretical reference for further exploration on insects olfactory recognition mechanism and formulation strategies on pests control and economic insects protection

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彭 竹,黄 丽,赵慧婷. 荧光竞争结合技术在昆虫嗅觉相关蛋白结合能力检测上的应用[J]. 激光生物学报. 2020, 29(2): 106-112
PENG Zhu, HUANG Li, ZHAO Huiting. Application of  Fluorescence Competitive Binding Technology in Detection  of the Binding Ability of Insects Olfactory Related Proteins[J]. Acta Laser Biology Sinica. 2020, 29(2): 106-112

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