功能性便秘是近几年比较常见的一种肠道疾病。越来越多的证据证明一些生物源功能活性物质对于治疗便秘有积极的疗效。螺旋藻富含多糖化合物，且具有广泛的生理功能。本文分析不同剂量螺旋藻多糖（PSP）对地芬诺酯诱导便秘小鼠的治疗效果，检测各组小鼠的体重、首次排便时间和数量、肠道酶活性，菌群组成以及相关功能基因表达等。结果显示，PSP低、中、高剂量组小鼠六小时内排便数量都显著增加（P<0.05），且首次排黑便时间缩短。同时，小鼠的体重增长没有受到显著影响。PSP治疗后，便秘小鼠肠道内木聚糖酶和蛋白酶活性恢复到正常组水平（P<0.05）。此外，PSP处理改变了便秘小鼠肠道主要微生物菌群组成，上调了一些有益功能基因的丰度，从而达到缓解便秘的效果。PSP处理上调KO0845（葡萄糖激酶）丰度水平，能维持机体血糖平衡。这些发现表明，PSP饲喂处理可显著改善小鼠肠道微生态，缓解小鼠便秘病症，为解决慢性便秘提供一种有效的食疗方案。

Functional constipation is a common intestinal disease in recent years. Increasing evidence shows that some biologically active substances has a positive effect on the treatment of constipation. Spirulina is rich in polysaccharide which have a number of physiological functions. This study was to investigate the therapeutic effects of polysaccharide from Spirulina platensis (PSP) on constipation mice induced by diphenoxylate. Different does of PSP were used to feed in the mice. Examinations on the mice included body weight, first defecation time and number, intestinal enzyme activity, bacterial composition and related functional gene expression. The results showed that the number of mouse defecations treatments increased significantly (P<0.05) within six hours, and the time for the first blackout was shortened in the low, medium and high dose groups of PSP.  At the same time, PSP had no significant effect on the growth of mouse body weight. After PSP treatment, the xylanase and protease activities in the intestine of constipation mice restored to levels in normal group (P<0.05). In addition, PSP treatment resulted in the changes in the composition of the main microflora in the intestinal tract of constipation mice, followed by upregulating the abundance of some beneficial functional genes and sequentially leading to the medication effect on constipation mice. Notably PSP treatment upregulates the abundance level of KO0845 (glucokinase) and maintains the body’s blood sugar balance. PSP feeding can significantly improve intestinal microecology, anesis constipation in mice and provide an effective diet for chronic constipation.