H1N1流感病毒属于常见的致病性病毒。流行病学调查研究表明，新生儿表型缺陷与流感病毒感染有关，但是具体机制还不明确。为了探讨H1N1流感病毒对胚胎发育的影响，本文通过流感病毒感染孕母鼠来构建流感病毒宫内感染的动物模型，分别在胚胎发育至E14.5、E15.5、E16.5、E17.5、E18.5 d以及出生1 d后测量各胚胎的体长，并在体视镜下观察胚胎体表各血管和器官来分析胚胎外表发育的情况，然后采用阿尔新蓝茜素红染色法观察各发育时期胚胎骨骼发育的情况。结果表明，流感病毒感染的小鼠胚胎的体长明显降低。外部器官发育的差异性可体现在眼、耳等器官，攻毒组成型稍晚，尾部异常卷曲更为严重。各骨骼发育攻毒组较对照组更迟缓，但没有出现长短肢或骨骼缺失等严重异常表型。本研究首次构建了流感病毒宫内感染的动物模型，探究了H1N1流感病毒对小鼠胚胎表型发育的影响。

The H1N1 influenza virus is a common pathogenic virus. Epidemiological analyses have shown that influenza viruses are associated with neonatal phenotypic defects, however the specific mechanisms are still unclear. In order to verify the effect of H1N1 influenza virus on embryonic development, an animal model of intrauterine infection of influenza virus was constructed by infecting pregnant mouse with influenza virus, and the embryos were developed to E14.5 days, E15.5 days, E16.5 days, respectively. The body length of each embryo were measured on E17.5 days, E18.5 days, and one day after birth. The blood vessels and organs of the embryo body were observed under a stereoscopic microscope to analyze the development of the embryo’s appearance, and then Alxin BlueAlizarin red staining was used to observe the development of embryonic bones at various developmental stages. The results showed that the body length of the mouse embryos in the transfecting group was significantly shortor than that in the control group, especially at the 17.5th and 18.5th days of development. The differences in the development of external organs can be reflected in the late anatomy of the eyes, ears and other organs, and the abnormal curling of the tail is more serious. The skeletal development was slower than the control group, but there were no serious abnormal phenotypes such as long legs or bone loss. In this research, an animal model of intrauterine infection of influenza virus was constructed for the first time, and the effect of H1N1 influenza virus on mouse embryo phenotype development was explored.