基于基因芯片技术的转Slnac2基因拟南芥差异表达基因分析

刘雪嫣,屈芳芳,王宏飞,李秋

激光生物学报 ›› 2019, Vol. 28 ›› Issue (6) : 535-542.

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激光生物学报 ›› 2019, Vol. 28 ›› Issue (6) : 535-542.
研究论文

基于基因芯片技术的转Slnac2基因拟南芥差异表达基因分析

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#br# Analysis of Differentially Expressed Genes in Transgenic Slnac2 Arabidopsis Based on Gene Chips

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摘要

NAC转录因子,对植物的生长发育及抵御逆境胁迫起着重要的作用。实验室前期克隆了辽宁碱蓬(Suaeda liaotungensis)Slnac2基因,转Slna2拟南芥(Arabidopsis thaliana)提高了抵御盐胁迫的能力。本研究利用基因芯片技术筛选转SlNAC2拟南芥差异表达基因,共筛选出差异表达2倍以上的基因1 258个。GO富集度分析结果显示,分子功能相关基因占34.06%,细胞组分相关基因占33.13%,生物学过程相关基因占32.81%。KEGG分析表明,差异表达基因涉及63个信号通路,主要有植物激素信号转导、核糖体、植物病原物相互作用和抗坏血酸代谢等。通过实时荧光定量PCR对部分差异基因进行验证, 所得结果与基因芯片结果一致。本研究揭示,SlNAC2可调控多个下游基因的表达,提高植物在逆境胁迫下的生存能力。

Abstract

NAC transcription factors play an important role in plant growth development and resistance to stress. In our previous studies, the Slnac2gene was cloned from Suaeda liaotungensis and transformed into Arabidopsis. SlNAC2 enhanced the salt tolerance of transgenic Arabidopsis. In this study, we screened the differentially expressed genes in transgenic Slnac2 Arabidopsis compared to the wild type by cDNA microarray. There are 1 258 genes which expressed more than twofold in transgenic Arabidopsis. The results of GO enrichment analysis showed that the molecular functionrelated genes accounted for 34.06%, the cellrelated genes accounted for 33.13%, and the biological processrelated genes accounted for 32.81%. KEGG(Kyoto Encyclopedia of Genes and Genomes pathways enrichment)analysis showed that 1 258 genes were involved in 63 different signaling pathways including plant hormone signal transduction, ribosome plantpathogen interaction, ascorbate and aldarate metabolism, etc. Some differential genes were verified by realtime PCR, and the results were consistent with those of the gene chip. In summary, SlNAC2 can regulate the expression of numerous downstream genes and improve plant viability under stress.

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刘雪嫣,屈芳芳,王宏飞,李秋. 基于基因芯片技术的转Slnac2基因拟南芥差异表达基因分析[J]. 激光生物学报. 2019, 28(6): 535-542
#br# Analysis of Differentially Expressed Genes in Transgenic Slnac2 Arabidopsis Based on Gene Chips[J]. Acta Laser Biology Sinica. 2019, 28(6): 535-542

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