花鲫早期体色发育和几个体色相关基因在不同鲫的表达分析

范云鹏,赵 韩,罗世民,唐玲倩,彭亮跃,刘文彬,肖亚梅,刘锦辉

激光生物学报 ›› 2019, Vol. 28 ›› Issue (4) : 343-352.

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激光生物学报 ›› 2019, Vol. 28 ›› Issue (4) : 343-352.
研究论文

花鲫早期体色发育和几个体色相关基因在不同鲫的表达分析

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Observation on Body Colour Development in Redwhite Crucian Carp and Expression Analysis of Pigment Genes in Different Color Crucian Carp

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摘要

红白花鲫胚胎发育期先观察到黑色素细胞的发生,孵化出膜后先后出现黄色素细胞、红色素细胞及虹彩细胞。1月龄花鲫幼苗体色呈浅青灰色,2月龄前后,花鲫皮肤黑色素细胞逐渐减少,体色发生“青转花”的变化,3月龄时基本形成红白镶嵌的成体体色。克隆获得了鲫Slc7a11和Pnp4a体色基因的全长cDNA。鲫Slc7a11全长1 782 bp,编码496个氨基酸,与斑马鱼Slc7a11基因氨基酸同源性达93.7%;鲫Pnp4a全长1 535 bp,编码291氨基酸,与斑马鱼Pnp4a基因氨基酸同源性达95.1%。RTPCR检测了Mitfa、Tyr、Slc7a11和Pnp4a在白鲫、红鲫、花鲫三种不同体色鲫胚胎及成体组织中的表达,分析结果显示:花鲫和红鲫一样有一个黑色素消退过程,鲫Slc7a11和Pnp4a体色基因与斑马鱼Slc7a11和Pnp4a具有高度同源性,花鲫成体皮肤组织也和红鲫成体皮肤组织一样检测到了Mitfa和Tyr的表达。Pnp4a在不同鲫的不同体色发育时期和不同组织都有表达,Slc7a11在不同鲫的不同体色发育时期均有表达,但在不同鲫的成体皮肤组织中均未表达。

Abstract

The early body color formation of redwhite crucian carp was observed. Melanocytes firstly appear before fertilization, then xanthophores happen and later iridophore appear. And then, the body colour of redwhite crucian carp changes from dark grey to redorange after about 3 months old. the fulllength cDNAs for Slc7a11 and Pnp4a were isolated. Our results revealed that the fulllength Pnp4a cDNA contains 1 535 bp, with an open reading frame of 783 nucleotides, which encodes a deduced protein of 291 amino acids, coding for a deduced sequence alignment showed that crucian carp Pnp4a shares high levels of identity with those from Danio rerio (95.1% ). The fulllength Slc7a11 cDNA contains 1 782 bp, with an open reading frame of 1488 nucleotides, which encodes a deduced protein of 496 amino acids. The amino acid sequence alignment demonstrated that the red crucian carp Slc7a11 protein shared a sequence identity of 93.7% with Danio rerio, Oryzias latipes.The expression of Mitfa,Tyr,Slc7a11 and Pnp4a in three kinds of different color crucian carp embryos and adult tissues were detected by RTPCR. The results showed that the redwhite crucian carp has the same melanin decay process as the red crucian carp.Amino acid sequence alignment showed that  Pnp4a and Slc7a11of red crucian carp share high identity with those from Cyprinus carpio.The adult skin tissue of the redwhite crucian carp was detected the expression of Mitfa and Tyr,so did the adult skin tissue of the red crucian carp.Pnp4a was expressed in different body color development stages and different tissues of different crucian carp.Slc7a11 was expressed in different body color development stages of different crucian carp. However,it was not detected in different adult skin tissues of different crucian carp.

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范云鹏,赵 韩,罗世民,唐玲倩,彭亮跃,刘文彬,肖亚梅,刘锦辉. 花鲫早期体色发育和几个体色相关基因在不同鲫的表达分析[J]. 激光生物学报. 2019, 28(4): 343-352
Observation on Body Colour Development in Redwhite Crucian Carp and Expression Analysis of Pigment Genes in Different Color Crucian Carp[J]. Acta Laser Biology Sinica. 2019, 28(4): 343-352

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