PEX5参与调控拟南芥根系抗逆性的初步研究

詹 爽,梁绮雯,罗为桂,肖 帅,苏 益,萧浪涛

激光生物学报 ›› 2019, Vol. 28 ›› Issue (4) : 336-342.

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PDF(3362 KB)
激光生物学报 ›› 2019, Vol. 28 ›› Issue (4) : 336-342.
研究论文

 PEX5参与调控拟南芥根系抗逆性的初步研究

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Roles of PEX5 in the Regulation of Stress Resistance in Arabidopsis Roots

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摘要

以拟南芥过氧化物酶体生成蛋白突变体pex5和野生型为试验材料,初步研究PEX5对拟南芥根系抗逆性的影响。结果表明,在MS基础培养基中,突变体pex5的根长相较于野生型根长显著变短;同时,拟南芥pex5突变体株高也低于野生型,说明PEX5基因功能缺失影响了拟南芥根系和茎的生长。突变体在添加氯化钠或甘露醇的MS培养基上培养时,萌发和根伸长受到的抑制显著高于野生型,突变体幼苗的SOD和POD活性也显著低于野生型,说明PEX5参与调控拟南芥根系的抗逆性。本研究为解析PEX5在植物抗逆中的调控机制提供了重要参考。

Abstract

The Arabidopsis peroxisome generate protein mutant pex5 and the wild type was utilized to study the roles of PEX5 in the stress resistance of Arabidopsis roots. The results showed that the root length of pex5 was significantly shorter than that of the wild type in MS medium. At the same time, the height of Arabidopsis pex5  mutant was also lower than that of the wild type, indicating that the deletion of PEX5 affected the root and stem growth of Arabidopsis. When the pex5  was cultured in MS medium with NaCl or mannitol, the inhibition of germination and root elongation was significantly stronger than that of the wild type, and the SOD and POD activity of the mutant seedlings was significantly lower than that of the wild type, which indicated that PEX5 affected the stress resistance of Arabidopsis roots. This study provides an important reference for understanding the regulation mechanism of PEX5 in plant stress resistance.

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詹 爽,梁绮雯,罗为桂,肖 帅,苏 益,萧浪涛.  PEX5参与调控拟南芥根系抗逆性的初步研究[J]. 激光生物学报. 2019, 28(4): 336-342
Roles of PEX5 in the Regulation of Stress Resistance in Arabidopsis Roots[J]. Acta Laser Biology Sinica. 2019, 28(4): 336-342

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