斑马鱼TAK1正向调控IRF7介导的天然免疫反应

彭 俊,晏 俊,张音音,冯 浩,肖 军

激光生物学报 ›› 2019, Vol. 28 ›› Issue (4) : 314-322.

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激光生物学报 ›› 2019, Vol. 28 ›› Issue (4) : 314-322.
研究论文

斑马鱼TAK1正向调控IRF7介导的天然免疫反应

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Zebrafish TAK1 Positively Regulates IRF7mediated Innate Immune Responses

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摘要

在鱼类中关于转化生长因子β 激活激酶1(TAK1)在天然免疫反应中的功能研究较少。为了探究TAK1在斑马鱼天然免疫中的功能,本文克隆并获得了一种斑马鱼tak1剪接异构体(Drtak1),其开放阅读框含有1 737个核苷酸,编码578个氨基酸,其中包括N端的丝氨酸/苏氨酸蛋白激酶结构域和C端的卷曲螺旋结构部。通过免疫荧光试验,证实DrTAK1是一种胞质蛋白。双荧光素酶报告试验显现在EPC细胞中单转DrTAK1不能诱导IFN的产生,但与IRF7共转时能显著提高其诱导干扰素启动子表达的能力。本文研究结果首次在斑马鱼中发现TAK1能正向调控IRF7介导的天然免疫反应,为后续DrTAK1功能研究奠定了基础。

Abstract

There are few reports on the function of transforming growth factor βactivated kinase 1(TAK1) in innate immune response in fish. To explore the role of zebrafish TAK1(Danio rerio TAK1, DrTAK1) in innate immune, a new splicing isoforms of Drtak1 has been cloned and characterized in this study. The open reading frame (ORF) of the Drtak1 has 1 737 nt, which can encode 578 amino acids. DrTAK1 includes a Nterminal serine/threonine protein kinases (S/TKc) and a C-terminal coiledcoil region. The results of immunofluorescence showed that DrTAK1 is a cytoplasmic protein. Overexpression of DrTAK1 in EPC cells downregulates the activity of IFN promoter in luciferase reporter assay in EPC cells. However when cotransferred with zebrafish IRF7, the DrTAK1 can obviously promote the IFNactivation ability of IRF7. The data in this study showed that the DrTAK1 positively regulated IRF7mediated innate immune responses for the first time in zebrafish, and it laid the foundation for subsequent DrTAK1 research.

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彭 俊,晏 俊,张音音,冯 浩,肖 军. 斑马鱼TAK1正向调控IRF7介导的天然免疫反应[J]. 激光生物学报. 2019, 28(4): 314-322
Zebrafish TAK1 Positively Regulates IRF7mediated Innate Immune Responses[J]. Acta Laser Biology Sinica. 2019, 28(4): 314-322

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