摘要
在鱼类中关于转化生长因子β 激活激酶1(TAK1)在天然免疫反应中的功能研究较少。为了探究TAK1在斑马鱼天然免疫中的功能,本文克隆并获得了一种斑马鱼tak1剪接异构体(Drtak1),其开放阅读框含有1 737个核苷酸,编码578个氨基酸,其中包括N端的丝氨酸/苏氨酸蛋白激酶结构域和C端的卷曲螺旋结构部。通过免疫荧光试验,证实DrTAK1是一种胞质蛋白。双荧光素酶报告试验显现在EPC细胞中单转DrTAK1不能诱导IFN的产生,但与IRF7共转时能显著提高其诱导干扰素启动子表达的能力。本文研究结果首次在斑马鱼中发现TAK1能正向调控IRF7介导的天然免疫反应,为后续DrTAK1功能研究奠定了基础。
Abstract
There are few reports on the function of transforming growth factor βactivated kinase 1(TAK1) in innate immune response in fish. To explore the role of zebrafish TAK1(Danio rerio TAK1, DrTAK1) in innate immune, a new splicing isoforms of Drtak1 has been cloned and characterized in this study. The open reading frame (ORF) of the Drtak1 has 1 737 nt, which can encode 578 amino acids. DrTAK1 includes a Nterminal serine/threonine protein kinases (S/TKc) and a C-terminal coiledcoil region. The results of immunofluorescence showed that DrTAK1 is a cytoplasmic protein. Overexpression of DrTAK1 in EPC cells downregulates the activity of IFN promoter in luciferase reporter assay in EPC cells. However when cotransferred with zebrafish IRF7, the DrTAK1 can obviously promote the IFNactivation ability of IRF7. The data in this study showed that the DrTAK1 positively regulated IRF7mediated innate immune responses for the first time in zebrafish, and it laid the foundation for subsequent DrTAK1 research.
彭 俊,晏 俊,张音音,冯 浩,肖 军.
斑马鱼TAK1正向调控IRF7介导的天然免疫反应[J]. 激光生物学报. 2019, 28(4): 314-322
Zebrafish TAK1 Positively Regulates IRF7mediated Innate Immune Responses[J]. Acta Laser Biology Sinica. 2019, 28(4): 314-322
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