gpr98基因突变与多种疾病相关，该基因在人类和斑马鱼中高度保守。建立斑马鱼gpr98 基因突变体稳定系，可为阐释 gpr98基因功能提供良好的动物模型和研究基础。本文利用CRISPR/Cas9基因敲除技术在斑马鱼gpr98基因2号外显子上选取两个相距42 bp的靶位点，分别体外合成sgRNA，并与Cas9 mRNA一起共注射至斑马鱼胚胎单细胞期的胚胎内。随机挑选发育72 h胚胎提取基因组DNA进行PCR分析，结果表明：除了有野生型DNA带外，部分胚胎有一条比野生型DNA小的带；进一步将F0代阳性个体与野生型的斑马鱼杂交，对杂交后代进行基因型分析，并成功筛选到缺失48 bp（Δ48 bp）的稳定遗传突变的gpr98基因敲除斑马鱼模型。该试验模型的构建为研究gpr98基因在心血管以及骨骼等组织器官的发育及相关疾病发生中的作用奠定了重要基础。

gpr98 is associated with a variety of diseases, and it is highly conserved in human and zebrafish. Constructing the gpr98 gene mutant strain of zebrafish can provide an excellent animal model and research basis for explaining the function of the gpr98 gene. In this paper, two target sites with a distance of 42 bp in exon 2 of zebrafish gpr98 gene were selected for sgRNA recognition. SgRNA was synthesized in vitro and coinjected with Cas9 mRNA into the embryo of zebrafish at 1cell stage. A few partial embryos were randomly selected for DNA genotyping when they are in 72hour stage. The results showed that in addition to the wildtype DNA bands, some embryos have a band smaller than the wildtype DNA bands. The F0 positive individuals were mated with wildtype zebrafish, and the offspring of the F0 generation positive individuals were tested for genotyping, and a stable genetic mutation strain with a deletion of 48 bp was obtained. The construction of the mutants model lays an important foundation for the study of the role of gpr98 gene in the development of cardiovascular, skeletal and other tissues and organs as well as the occurrence of related diseases.