采用石英晶体微天平（QCM）非侵入式实时监测乳鼠原代心肌细胞于金晶体电极上的动态黏附响应与不同浓度肌力药物下的粘弹性响应。在乳鼠原代心肌细胞黏附铺展于金晶体电极表面后，加入不同浓度的正性肌力药物异丙肾上腺素与负性肌力药物维拉帕米，监测QCM频率(F)以及动态电阻(R)的实时变化，采用粘弹性指数CVI（CVI=ΔR/ΔF）表征细胞的粘弹性变化，并通过光学显微镜观察药物作用下细胞的形态变化。结果表明：随着异丙肾上腺素浓度的增加，药物引起的QCM频率下降、电阻增加与CVI增大的幅度均增大；光学显微镜下观测到细胞收缩，符合CVI变大、细胞变硬的响应；随着维拉帕米浓度的增加，药物引起的QCM频率升高、电阻下降与CVI减小的幅度均增大；光学显微镜下观测到细胞舒张，符合CVI变小、细胞变软的响应。说明QCM与原代心肌细胞结合作为心血管药物筛选细胞模型与工具有很大的应用前景。

The quartz crystal microbalance (QCM)was used to monitor the dynamic adhesions of neonatal rat primary cardiomyocytes and the subsequent viscoelastic changes induced by different concentrations of inotropic drugs onto the gold crystal electrodes in a noninvasive and realtime manner. The adhesion of neonatal rat primary cardiomyocytes onto gold electrode surface, and the subsequent drug effects of different concentrations of cardiomyocyte positive inotropic drug isoprenaline and cardiomyocyte negative inotropic drug verapamil, were monitored through the frequency (F)and motional resistance (R)measurements of the QCM in a realtime way. Cell viscoelastic index CVI (CVI=ΔR/ΔF)was used to characterize the viscoelastic changes of the cells, and the druginduced morphological changes of the cells were observed by optical microscope.  The results showed that with the increase of isoprenaline concentration, the magnitudes of QCM frequency drop, R increase and CVI increase all increased; whereas with the increase of verapamil concentration, the magnitudes of QCM frequency increase, drops in both R and CVI all increased. Cells’ contraction under the treatment of isoprenaline was observed under optical microscope, which is consistent with the result of increased CVI indicating that the cells became stiffer; while cells’ relaxation under the treatment of verapamil was observed under optical microscope, which is consistent with the result of decreased CVI indicating that the cells became softer. Our results suggest that the QCM has a great application potential as a tool in cardiovascular drug screening and evaluations.