精子密度仪在哺乳动物中已推广应用，但在家禽中还研究很少。本文以黑凤鸡和攸县麻鸭精液为实验材料，手持精子密度仪与血细胞计数板为检测工具，对影响精子密度测定方法准确率因素进行分析，建立鸡、鸭精子密度吸光度函数并进行验证。结果表明：用密度仪测定时的稀释液（简称“测试液”）为3%NaCl时，精液稀释后应尽快检测吸光度；样液在比色皿中的混匀方式对吸光度测定有很大影响；精液用3%NaCl以及3种常用家禽精液稀释液进行10倍稀释后测定吸光度，B液吸光度显著高于与其它3组（P＜0.05），其它3组间差异不显著（P>0.05）。用测试液为3.0%NaCl，鸡和鸭精子密度吸光度呈三次函数关系，回归方程分别是Y₁=1.374X³-0.786X²+0.945X-0.002（R²=0.997）、Y₂=1.283X³-0.899X²+0.994X-0.009（R²=0.996）；用0.9%NaCl代替3%NaCl作测试液简化精子密度测定方法可行，鸡精子密度吸光度回归方程为Y₃-0.264X³+1.23X²+0.468X+0.019 （R²=0.999)。鸡精液测试液为0.9%NaCl、3%NaCl时两种函数的吸光度最佳范围分别是0.035~0.692、0.069~0.624，在此范围内计数板与方程两种方法得到的密度差异不显著（P>0.05），以计数法为真实值，两种方法平均相对误差分别为6.95%、3.11%。以3%NaCl为测试液，鸭精液函数所测样品吸光度最佳范围小于鸡精液的，以计数板法为真实值，在吸光度0.054~0.123范围内的，函数与计数板两种方法所得的精子密度的平均相对误差为4.61%。本文将促进家禽手持精子密度仪研发，以及更好的使用哺乳动物精子密度仪。

Sperm densitometer has been widely applied in mammals, whereas little research has been done in poultry. Taking Black Silkies chicken and Youxian Partridge Duck semen as experimental materials, the sperm densitometer and blood cell counting board were used as testing tools,  the factors affecting the determination accuracy rate were analyzed, and the roosters and drakes sperm absorbancedensity curve were established and verified. The results showed that the absorbance should be measured as soon as possible after the semen was diluted using a diluent of 3%NaCl. There was great influence on the absorbance measurement when the original semen was diluted by different mixing method. The absorbance of B liquid is significantly higher than that of other 3 groups（P＜0.05)when the same semen was diluted 10 times with 3%NaCl and other three kinds of common semen dilutions, and there were no significant differences within the other 3 groups （P>0.05). Using the 3.0%NaCl as diluents, the relationship of the absorbancedensity curve was cubic function for the rooster and ducks semen, and the  regression equations were Y₁=1.374X³-0.786X²+0.945X-0.002（R²=0.997）and Y₂=1.283X³-0.899X² +0.994-0.009（R²=0.996）.It was feasible to simplify the sperm density determination methods using 0.9%NaCl instead of 3%NaCl, and the regression equations was Y₃=-0.264X³+1.23X²+0.468X+0.019 （R²=0.999). The optimal range of absorbance for the curve of roosters semen 0.9%NaCl and 3%NaCl was between 0.035~0.692 and 0.069~.624. And as for 3%NaCl drakes semen, it might be less than that of 3.0%NaCl roosters semen. There was no significant difference between the cell count and the curve calculated density within each optimized range （P>0.05). The average relative error of the 0.9%NaCl and 3%NaCl was 6.95%and 3.11%when using the counting value as the true one which is within each  optimized absorbance range, and that of 3%NaCl darkes semen was 4.61%with the absorbance range of 0.054~0.123. This paper will promote the research and development of handheld sperm densitometer for poultry and better use of mammalian sperm densitometer.