本文研究了不同碳源对须糖多孢菌生长以及丁烯基多杀菌素生物合成的影响，通过寻找优势碳源优化发酵培养基配方，促进须糖多孢菌丁烯基多杀菌素的生物合成。试验共设11个处理，1个对照，通过单因素试验比较不同处理组菌体OD600值和丁烯基多杀菌素产量，筛选获得最优碳源及其发酵培养基配方。结果表明，除可溶性淀粉和木糖外，须糖多孢菌在9种碳源中都能进行生长，对不同构型碳源显示较好的利用率。在以半乳糖、葡萄糖、果糖和甘露糖作为碳源时具有较好的生长速率，而以甘露糖为碳源时能显著促进丁烯基多杀菌素的合成。选择甘露糖最佳添加浓度为5 g/L，须糖多孢菌最高菌体浓度和丁烯基多杀菌素产量分别是初始配方条件的1.32倍和1.78倍，显著提高了丁烯基多杀菌素的产量。上述结果为培养基碳源对丁烯基多杀菌素生物合成影响机制的研究及丁烯基多杀菌素大规模工业化发酵生产提供了科学依据和新的技术途径。

In this research, the effects of different carbon sources on the strain growth and butenylspinosyn biosynthesis of Saccharopolyspora pogona were studied. The study was aimed to improve the butenylspinosyn biosynthesis by looking for dominant carbon sources and optimizing the fermentation medium formula. A total of eleven processing groups and one control group were set up in this experiment. The strain OD600 value and butenylspinosyn production in different processing groups were compared by single factor experiment for screening the optimal carbon source and fermentation medium formulation. The results showed that Saccharopolyspora pogona could grow in 9 kinds of carbon sources except for xylose and soluble starch, and exhibited good utilization rate for different types of carbon sources. Saccharopolyspora pogona has a good growth rate when using galactose, glucose, fructose and mannose as carbon sources, but just mannose can promote butenylspinosyn biosynthesis. The 5 g/L mannose was selected as the optimal concentration and it was found that Saccharopolyspora pogona strain concentration and butenylspinosyn production were 1.32 times and 1.78 times of the initial conditions, respectively. Its production significantly increased. The study provides a scientific bases and a new technical approach for the influencing mechanism study of medium carbon sources on the butenylspinosyn biosynthesis and the largescale industrial production of butenylspinosyn.