线粒体自噬,是指通过选择性的识别并清除损伤、衰老及功能紊乱的线粒体，对维持细胞内线粒体质量和数量的平衡产生了重要作用。与动物和酵母中线粒体自噬的研究进展相比，植物线粒体自噬的途径及具体调控机制尚不明确。基于GFP标签，本文探究了氧化胁迫下植物线粒体自噬发生情况。研究发现甲基紫精诱导线粒体在液泡中积累，并呈现两种状态：1）GFP小体包含的线粒体；2）不含GFP的线粒体。本研究发展的GFP标签策略可为植物线粒体自噬关键调控因子的筛选提供借鉴。

Mitophagy plays an important role in maintaining the balance between mitochondrial quality and quantity in cells by selectively identifying and removing damaged, aged and dysfunctional mitochondria. Compared with the research progresses in mammal and yeast, the regulatory mechanisms of mitophagy in plant are still unclear. Based on a GFP tag, this work investigated the occurrence of mitophagy in Arabidopsis upon oxidative stress. We found that methyl viologen could induce mitochondria accumulated in vacuole, showing the following two patterns:1)mitochondria contained in GFP bodies;2)mitochondria only. The GFP tag strategy can provide reference for the selection of key regulatory factors of mitophage in plants.