Wnt16属于非经典wnt信号途径上的一员，目前有大量的研究表明wnt16与胚胎的血液发育和骨骼发育相关。为了阐明wnt16在造血过程中的调控机制，利用RTPCR（逆转录PCR）扩增斑马鱼wnt16基因的ORF（开放阅读框）序列全长，构建真核表达载体pCAGGSP7/wnt16，测序正确后，用该表达载体免疫BALB/c雌鼠，进行DNA免疫，制备斑马鱼抗WNT16特异性多克隆抗体。结果表明：通过DNA免疫制备的斑马鱼抗WNT16抗血清能特异性识别WNT16蛋白。斑马鱼WNT16抗体成功制备为进一步的功能研究奠定了重要基础。

Wnt16 is a member of the nonclassical wnt signaling pathway. Recently, a large number of studies have shown that wnt16 is related to the development of hematopoiesis and skeleton in zebrafish. To elucidate the mechanism of wnt16 in regulation of hematopoiesis, the whole ORF (open reading frame) sequence of wnt16 gene in zebrafish was amplified by RTPCR (reverse transcription PCR), and the eukaryotic expression vector pCAGGSP7/wnt16 was constructed. Then BALB/c female mice immunized with the expression vector to generate antiWNT16 specific polyclonal antibody in zebrafish. The results showed that the antiWNT16 antiserum prepared by immunizing DNA could specifically recognize WNT16 protein. The successful preparation of zebrafish WNT16 antibody lays an important foundation for further study.