本文通过溶胶-凝胶法制备了Fe-TiO²和Ni-TiO²纳米颗粒，并研究这两种纳米颗粒体外光动力疗法（PDT）对HL60细胞的灭活效果。通过透射电镜（TEM）、能谱仪（EDS）、X射线衍射（XRD）、紫外可见光（UVVis）吸收光谱等方法对纳米颗粒进行表征。使用CCK8法分别测定Fe-TiO²和Ni-TiO²对HL60细胞的灭活效果。结果表明，不同终值浓度及掺杂量的Fe-TiO²和Ni-TiO²纳米颗粒对HL60细胞的暗毒性较低，但是PDT效率均显著高于未掺杂的TiO²。在各自的最佳作用参数下，PDT灭活效率分别达到72.5%±1.6%和56.4%±1.2%。此外，还对这两种纳米颗粒灭活效果的差异进行了探讨。

In this paper, Fe-TiO² and Ni-TiO² nanoparticles were synthesized by solgel method. The inactivation of HL60 cells in vitro photodynamic therapy was studied. The nanoparticles were characterized by transmission electron microscope (TEM), energy dispersive spectrometer(EDS), Xray diffraction (XRD), ultravioletvisible (UVVis) absorption spectrum etc. The inactivation of HL60 cells by Fe-TiO² and Ni-TiO² was analyzed by CCK-8 assay. The experimental results show that Fe-TiO² and Ni-TiO² nanoparticles with different final concentration and doping amount have lower dark toxicity to HL60 cells, but the PDT efficiency is significantly higher than that of pure TiO². Under the optimal parameters, the PDT inactivation efficiency reached 72.5%±1.6% and 56.4%±1.2% respectively. In addition, the differences in the inactivation effects of these two nanoparticles were also discussed.