应用高速逆流色谱（HSCCC）进行了雨生红球藻中虾青素的分离制备工艺优化，结果最优条件为正己烷∶乙酸乙酯∶乙醇∶水（6.5∶5∶6.5∶3，v/v/v/v）作为两相溶剂系统，以下相为固定相，上相为流动相，转速850 r/min，流速3 mL/min，温度25  ℃，上样浓度10 mg/mL，上样量10 mL。进一步应用高效液相色谱、质谱并与标准品比对，对所得虾青素进行鉴定。本文的研究结果为应用HSCCC高效制备雨生红球藻虾青素提供了技术支持。

Highspeed countercurrent chromatography(HSCCC)was developed for separation and purification of astaxanthin from Haematococcus pluvialis.The optimized twophase solvent system, revolution speed, flow rate, temperature, sample concentration and sample volume were nhexaneethyl acetateethanolwater(6.5∶5∶6.5∶3, v/v/v/v), 850 r/min, 3 mL/min, 25  ℃, 10 mg/mL and 10 mL, respectively. The lower phase was used as stationary phase, while the upper phase was the mobile phase of HSCCC. Furthermore, the compound collected from HSCCC analyzed by high performance liquid chromatography(HPLC), mass spectrometry(MS)and standard and was confirmed as astaxanthin. This paper can provide technical support to efficient preparation of astaxanthin from H. pluvialis using HSCCC.