目的：建立商陆离体再生体系。方法：选取商陆的幼茎、茎节、叶片、叶柄和顶芽为外植体，以MS作为基本培养基，通过添加不同浓度配比的植物生长调节剂分别进行愈伤组织、丛生芽和生根诱导，筛选商陆离体再生体系方案。结果：顶芽和幼茎为外植体诱导的愈伤组织出愈时间早，愈伤组织质量高，以培养基MS+6BA 0.5 mg/L+2.4-D 0.5 mg/L的诱导率最高，达到100%；其中，只有以顶芽产生的愈伤组织才能分化出丛生芽，芽分化培养基为MS+6-BA 2.0 mg/L+NAA 0.25 mg/L，诱导率为98%；诱导生根的适宜培养基为1/2 MS+NAA 0.3 mg/L，诱导率达100%。结论：建立和完善了商陆离体再生体系方案，为商陆遗传转化体系的构建奠定了基础。

Objective:The aim is to establish regeneration system in vitro of Phytolacca acinosa Roxb. Methods: To screen regeneration system scheme of Phytolacca acinosa Roxb, young stem and stem node and leaves and petioles and apical buds of Phytolacca acinosa Roxb were chosen as explants, and MS was used as basic medium with different plant growth regulators combinations to induce callus and buds and roots. Results: Callus could be induced from apical buds and young stem with early emergence and high quality, and the callus induction rate reached 100% in medium MS+6-BA 0.5 mg/L+ 2.4-D 0.5 mg/L. Among them, only the callus produced from apical bud could differentiate cluster buds. The medium for bud differentiation was MS+6-BA 2.0 mg/L+NAA 0.25 mg/L, and the induction rate reached 98%. The suitable medium for rooting was 1/2 MS + NAA 0.3 mg/L with 100% induction rate. Conclusion:The regeneration system in vitro of Phytolacca acinosa Roxb was established and perfected, which laid a foundation for the construction of genetic transformation system in Phytolacca acinosa Roxb.