目的：制备分泌特异性抗人甲状腺球蛋白（thyroglobulin,Tg）单克隆抗体的杂交瘤细胞株，为建立高灵敏度的Tg检测方法做准备。方法：以天然人源甲状腺球蛋白为抗原经皮下免疫BALB/c小鼠，通过细胞融合制备分泌抗人甲状腺球蛋白单克隆抗体，并对其进行特异性鉴定，建立检测Tg的双抗体ELISA(enzymelinked immunosorbent assay)夹心法。结果：获得7株可稳定分泌抗人甲状腺球蛋白单克隆抗体的杂交瘤细胞株，经ELISA鉴定，筛选抗体可与Tg抗原有良好的特异性反应。建立的双抗体夹心ELISA方法敏感性可达1 ng/mL。 结论：成功制备了抗人Tg单克隆抗体并建立了检测人Tg双抗体夹心ELISA方法，为进一步研发Tg快速诊断试剂盒提供了原料。

Objective: To prepare monoclonal antibody against human thyroglobulin and establish a highly sensitive method to detect human thyroglobulin. Methods: BALB/c mice were subcutaneously immunized by native human thyroglobulin, the splenocytes of immunized mice were fused with SP2/0 myeloma cells by a routine method. The hybridoma cells secreting specific antibody were detected. The stability of the obtained hybridoma cells and the specificity of antithyroglobulin monoclonal antibody the hybridoma cells secreted were identified. Based on all above, the double antibody sandwich ELISA method was established to detect thyroglobulin. Results: Seven hybridoma cell lines which could secrete monoclonal antibody stably were obtained, and ELISA indicated the antibodies had a good specificity and sensitivity to Tg antigen. A sandwich ELISA kit for detecting human thyroglobulin was established, and its sensitivity reached to 1 ng/mL. Conclusion: The antithyroglobulin monoclonal antibodies were prepared and a sandwich ELISA kit for the detection of human thyroglobulin was established successfully, which would provide a raw material for the development of Tg rapid diagnostic kits.