大豆Lea5基因是LEA基因家族成员之一。为分析大豆Lea5基因的功能，构建了大豆Lea5基因植物过表达载体pBI121Lea5。通过农杆菌介导法转化烟草,获得TL06、TL09、TL17和TL32等4个转大豆Lea5基因烟草株系。RTPCR分析表明大豆Lea5基因在4个转基因烟草株系的T2代植株均有不同程度的表达。以转基因TL09和TL32株系T2代植株为材料，进行了干旱和盐渍处理，结果表明，2个转大豆Lea5基因烟草株系T2代植株对干旱和盐渍的抗性显著强于野生型烟草，说明大豆Lea5基因可以提高植物抗干旱和盐碱的能力。

In order to investigate biological function of soybean Lea5 gene, one of LEA gene family, the overexpression vector of pBI121Lea5 was constructed. Through leaf discs transformation by Agrobacterium (LB4404:pBI121Lea5), four transgenic plants of TL06, TL09, TL17 and TL32 were selected and identified. Analysis by RTPCR shows that soybean Lea5 gene was expressed in T2 generation plants of the four transgenic lines in different level. Treated with drought and salinity, T2 generation plants of TL09 and TL32 displayed more resistance to drought and more tolerance to salinity. This indicates that overexpression of soybean Lea5 gene confers resistance to drought and tolerance to salinity in plant.